IrO_(2)@SF纳米粒子的合成及其抗肿瘤应用

Synthesis of IrO_(2)@SF Nanoparticle and Its Antitumor Applications

缺氧肿瘤微环境通常会促进肿瘤增殖和导致肿瘤细胞对活性氧(ROS)治疗的抵抗.选择丝素蛋白(SF)为模板,通过模拟生物矿化作用在SF表面矿化一层氧化铱(IrO_(2)),得到丝素蛋白和氧化铱的纳米复合物IrO_(2)@SF(IOS).天然SF的加载赋予了IOS多重抗肿瘤活性.实验结果表明:IOS具有较高的光热转换效率、过氧化氢酶和过氧化物酶活性以及较好的声动力效果.IOS能够通过分解肿瘤微环境过表达的过氧化氢,从而逆转肿瘤微环境的缺氧状态.体外细胞实验表明:IOS实现了光热联合纳米酶催化动力学抗肿瘤与声动力联合纳米酶催化动力学抗肿瘤治疗,2种多模式结合的治疗方式均展现了优异的抗肿瘤效果.同时,由于SF这种天然蛋白的加载,IOS还具有较高的生物相容性.

Hypoxic tumor microenvironment usually promotes tumor proliferation and leads to the resistance of tumor cells to reactive oxygen species(ROS)therapy.In this paper,silk fibroin(SF)was selected as the template,and a layer of iridium oxide(IrO_(2))was mineralized on the surface of SF by biomineralization.A nanocomplex of IrO_(2)@SF nanoparticles(IOS)was obtained.The loading of natural SF endowed the IOS with multiple antitumor activities.The results show that the IOS have high photothermal conversion efficiency,catalase and peroxidase activities,and promoted sonodynamic effect.IOS can reverse the hypoxic state of tumor microenvironment by decomposing excess hydrogen peroxide.Cell experiments(in vitro)show that IOS achieves two antitumor therapeutic modes,including photothermal therapy incorporated with nanocatalytic therapy,and sonodynamic therapy incorporated with nanocatalytic therapy.Both treatment modalities show excellent antitumor effects.Meanwhile,due to the loading of native SF,IOS also has high biocompatibility.This study provides an effective and reliable treatment regimen for antitumor therapy.