赶黄草总黄酮对铁超载大鼠铁负荷影响的初步研究

Preliminary Study on the Effect of Total Flavonoids of Penthorum chinense Pursh on Iron Loading ofIron Overload Rats

目的:探讨赶黄草总黄酮对铁超载模型大鼠铁负荷的影响。方法:雄性SD大鼠随机分为对照组、模型组、赶黄草总黄酮低(25 mg·kg^(-1))、高(100 mg·kg^(-1))剂量组。腹腔注射右旋糖酐铁诱导铁超载大鼠模型,同时给予赶黄草总黄酮干预,火焰原子吸收光谱法测定各组大鼠肝、脾、胰腺、心脏和肾脏的铁含量。结果:与对照组相比,模型组大鼠肝、脾、胰腺、心脏和肾脏铁含量均显著升高(P <0.001),铁超载大鼠模型造模成功;赶黄草总黄酮干预后各组织铁含量均显著低于模型组(P <0.01),赶黄草总黄酮高剂量组肝脏、肾脏、心脏铁含量显著低于低剂量组(P <0.05)。结论:赶黄草总黄酮能够减轻铁超载大鼠组织铁负荷,这可能与其具有较强的铁螯合活性和抗氧化特性有关。

Objective:To investigate the effect of total flavonoids of Penthorum chinense Pursh(PCP)on iron loading in rats of iron overload model.Method:Male SD rats were randomly divided into a control group,a model group,and a low(25 mg·kg-1)and high(100 mg·kg-1)dose group of total flavonoids of PCP.Iron overload was induced by intraperitoneal injection of iron dextrose in the rat model,and the iron content in liver,spleen,pancreas,heart and kidney of rats in each group was determined by flame atomic absorption spectrometry.Result:Compared with the control group,the iron content in the liver,spleen,pancreas,heart and kidney of rats in the model group was significantly increased(P<0.001),and the iron overload rat model was successful;the iron content in all tissues was significantly decreased than that in the model group after the intervention of total flavonoids of PCP(P<0.01),and the iron content in the liver,kidney,and heart of total flavonoids of PCP was significantly lower than that of the low dose group in the high dose group(P<0.05).Conclusion:The total flavonoids of PCP were able to reduce tissue iron loading in iron-overloaded rats,which may be related to its strong iron chelating activity and antioxidant properties.