UPLC-MS/MS法测定黄瓜中肟菌酯及其代谢物肟菌酸残留量

Determination of Residual Levels of Trifloxystrobin and Its Metabolite Trifloxystrobin Acid in Cucumissativus by UPLC-MS/MS Method

采用QuEChERS技术对黄瓜样品进行萃取,经C18净化和BEH C18色谱柱分离,采用超高效液相色谱-串联质谱法(Ultra-High Performance Liquid Chromatography-Tandem Mass Spectrometry,UPLC-MS/MS),测定黄瓜中肟菌酯及其代谢物肟菌酸残留量。结果表明,在1.0~50.0μg·L^(-1),肟菌酯及其代谢物肟菌酸的质量浓度与信号响应的峰面积具有良好的线性关系,相关系数R^(2)≥0.9997;肟菌酯及其代谢物肟菌酸的检出限为0.05~0.08μg·kg^(-1),定量限为0.2~0.3μg·kg^(-1);加标回收率为87.3%~101.7%,相对标准偏差为1.1%~3.2%。

QuEChERS technology is utilized to extract the Cucumis sativus samples are extracted by QuEChERS technique,and purified by C18 purification and separated by BEH C18 chromatography column,ultra-high performance liquid chromatography-tandem mass spectrometry is used to determine the residual levels of trifloxystrobin and its metabolite trifloxystrobin acid in Cucumis sativus.The results show that in the range of 1.0~50.0μg·L^(-1),the mass concentration of trifloxystrobin and its metabolite trifloxystrobin acid had a good linear relationship with the peak area of the signal response,and the correlation coefficient(R^(2))is 0.9997 or higher.The limit of detection of trifloxystrobin and its metabolite trifloxystrobin acid is 0.05~0.08μg·kg^(-1),and the limit of quantitation is 0.2~0.3μg·kg^(-1).The standard recovery is 87.3%~101.7%,the relative standard deviation is 1.1%~3.2%.