两种同属食药同源植物黄精与玉竹代谢组学比较分析

Comparative metabolomics analysis between two congeneric edible and medicinal plants,Polygonatum sibiricum Red.and Polygonatum odoratum(Mill.)Druce

采用代谢组学方法比较分析了同属食药同源植物黄精和玉竹代谢物质的组成及差异。利用基于超高效液相色谱-质谱联用技术(UHPLC-MS)的非靶向代谢组学技术,对黄精与玉竹的根部进行了分析。两种中药共检测到583个差异代谢物,玉竹与黄精相比,其中共有536个代谢物含量显著上升,47个代谢物含量显著下降。在这些代谢物中,有150种黄酮类化合物在黄精和玉竹中存在显著性差异,其中:139种显著上调,11种显著下调。甾体皂苷类化合物在黄精和玉竹中共检测到110种,其中:93种显著上调,17种显著下调。生物碱类化合物在黄精和玉竹中共检测到73种,其中:61种显著上调,12种显著下调。98种酚酸类化合物在黄精与玉竹中存在显著性差异,其中:96种显著上调,2种显著下调。48种萜类化合物在黄精与玉竹中存在显著性差异,其中:47种显著上调,1种显著下调。这些研究结果可为黄精与玉竹的开发研究提供理论依据。

Metabolomics methods were used to comparatively analyze the composition and differences of the metabolites between two congeneric edible and medicinal plants of Polygonatum sibiricum Red.and Polygonatum odoratum(Mill.)Druce.The roots of P.sibiricum and P.odoratum were analyzed by a non-targeted metabolomics technique based on ultrahigh performance liquid chromatography-mass spectrometry(UHPLC-MS).A total of 583 significantly different metabolites were detected from the two medicinal plants,of which 536 increased dramatically in P.odoratum compared to P.sibiricum,while 47 reduced considerably in P.odoratum.Among these metabolites,there were significant differences in 150 flavonoids between P.sibiricum and P.odoratum,of which 139 increased greatly and 11 significantly decreased.A total of 110 steroid saponins were detected in P.sibiricum and P.odoratum,of which 93 were significantly increased and 17 were significantly decreased.A total of 73 alkaloids were detected in P.sibiricum and P.odoratum,of which 61 were significantly in⁃creased and 12 were significantly decreased.There were significant differences between 98 terpenoids in P.sibiricum and P.odoratum,of which 96 were significantly increased while 2 were significantly decreased.There were significant differ⁃ences between 48 terpenoids in P.sibiricum and P.odoratum,of which 47 were significantly increased and only 1 was significantly decreased.These research results would provide some theoretical basis for the medical development and appli⁃cation of P.sibiricum and P.odoratum.