圈养野生动物粪便及生境耐药菌分布状况研究

Distribution of Resistant Bacteria in Captive Wild Animals and Habitats

为探究圈养野生动物粪便及生境抗生素耐药菌分布情况,该研究利用不同抗生素选择性平板采集7种野生动物粪样和生境水土样共54份样本,进行耐药菌的筛选和检出率计算与分析。结果显示,野生动物周围环境样本耐药菌检出率与粪样耐药菌检出率统计结果基本类似,其中粪样耐药菌检出率介于0~73.36%之间,生境中水样耐药菌检出率介于0~65.69%之间,土样耐药菌检出率介于0~82.47%之间。野生动物粪样存在不同程度细菌耐药现象,并有明显多重耐药性。野生动物中氨苄青霉素耐药性最为严重,其次为氟苯尼考抗生素。该研究可为中国野生动物自然环境细菌耐药性监测工作奠定基础。

To explore the distribution of antibiotic-resistant bacteria in captive wild animals and habitats,a total of 54 fecal samples and habitat samples were collected from 7 wild animals for the detection and analysis of antibiotic resistant bacteria by different antibiotics selective tablet.The results showed that wild animal fecal samples of drug-resistant bacteria detection rate is between 0~73.36%.Habitat in water samples of drug-resistant bacteria detection rate between 0~65.69%,soil samples of drug-resistant bacteria detection rate between 0~82.47%.Wild animal fecal samples are different degree of bacterial drug resistant,and have obvious multiple drug resistance.The detection rate of wildlife habitat sample drug-resistant bacteria is similar with the detection rate of fecal samples drug-resistant bacteria.Ampicillin resistance is most serious in wildlife,followed by florfenicol.The study would lay the foundation for surveillance of natural environment bacterial drug resistance.