戊酸钠对脂多糖诱导急性肠炎损伤小鼠的治疗作用

Therapeutic Effects of Sodium Valerate on Lipopolysaccharide⁃Induced Acute Enteritis Injury in Mice

本试验旨在探讨戊酸钠对脂多糖(LPS)诱导急性肠炎损伤小鼠的治疗作用。试验选用24只体重相近的6周龄雄性C57BL/6J小鼠,随机分为3组,分别为对照组(CON组)、脂多糖组(LPS组)和戊酸钠组(SV组),每组8只。LPS组和SV组小鼠腹腔注射2.5 mg/kg BW的LPS,CON组腹腔注射相同体积的生理盐水;24 h后,CON组和LPS组小鼠自由饮食和饮水,SV组每天灌喂100 mg/kg BW的戊酸钠0.2 mL,连续7 d。结果表明:与CON组相比,LPS组小鼠结肠长度显著降低(P <0.05),血清白细胞介素-1β(IL-1β)含量和脾脏指数显著提高(P <0.05),结肠乙酸、丙酸、丁酸、戊酸和总短链脂肪酸含量显著降低(P <0.05),结肠拟杆菌门(Bacteroidota)相对丰度显著提高(P <0.05),结肠毛螺菌科NK4A136群(Lachnospiraceae_NK4A136_group)相对丰度显著降低(P<0.05)。与LPS组相比,SV组小鼠结肠长度显著提高(P<0.05),血清IL-1β和肿瘤坏死因子-α(TNF-α)含量以及脾脏指数显著降低(P<0.05),结肠乙酸、戊酸和总短链脂肪酸含量显著提高(P<0.05),结肠阿克曼菌属(Akkermansia)相对丰度显著降低(P<0.05),结肠脱硫弧菌科未分类属(Desulfovibrionaceae_unclassified)相对丰度显著提高(P<0.05)。与CON组相比,SV组小鼠结肠菌群Shannon指数和Simpson指数显著提高(P<0.05),结肠阿克曼菌属相对丰度显著降低(P<0.05),结肠副拟杆菌属(Parabacteroides)相对丰度显著提高(P<0.05)。综上所述,戊酸钠可以通过降低LPS诱导急性肠炎损伤小鼠血清炎症因子含量,提高结肠SCFA含量,并改善结肠微生态平衡,从而发挥其对急性肠炎的治疗作用。

The purpose of this study was to investigate the therapeutic effects of sodium valerate on lipopolysac⁃charide(LPS)⁃induced acute enteritis injury in mice.Twenty⁃four 6⁃week⁃old male C57BL/6J mice with simi⁃lar body weight were randomly divided into 3 groups as control group(CON group),lipopolysaccharide group(LPS group)and sodium valerate group(SV group),with 8 mice in each group.Mice in LPS group and SV group were intraperitoneally injected with LPS 2.5 mg/kg BW,while mice in CON group were intraperitoneal⁃ly injected with the same volume of normal saline.After 24 h,mice in CON group and LPS group were fed di⁃et and water freely,while mice in SV group were fed 0.2 mL 100 mg/kg BW sodium valerate every day for 7 consecutive days.The results show that compared with CON group,the colon length in mice in LPS group was significantly decreased(P<0.05),the serum interleukin⁃1β(IL⁃1β)content and spleen index were signifi⁃cantly increased(P<0.05),the contents of acetic acid,propionic acid,butyric acid,valeric acid and total short⁃chain fatty acid in colon were significantly decreased(P<0.05),the Bacteroidota relative abundance in colon was significantly increased(P<0.05),and the Lachnospiraceae_NK4A136_group relative abundance in colon was significantly decreased(P<0.05).Compared with LPS group,the colon length in mice in SV group was significantly increased(P<0.05),the serum contents of IL⁃1βand tumor necrosis factor⁃α(TNF⁃α)as well as spleen index were significantly decreased(P<0.05),the contents of acetic acid,valeric acid and total short⁃chain fatty acid in colon were significantly increased(P<0.05),the Akkermansia relative abundance in colon was significantly decreased(P<0.05),and the Desulfovibrionaceae_unclassified relative abundance in colon was significantly increased(P<0.05).Compared with CON group,the Shannon index and Simpson in⁃dex of colonic microflora in mice in SV group were significantly increased(P<0.05),the Akkermansia rela⁃tive abundance in colon was significantly decreased(P<0.05),and the Parabacteroides relative abundance in colon was significantly increased(P<0.05).In conclusion,the sodium valerate can reduce the serum inflam⁃matory factor contents in mice with LPS⁃induced acute enteritis injury,increase the SCFA content in colon,and improve the microbiota balance in colon,so as to exert its therapeutic effect on acute enteritis.