冰片提神雾化液对小鼠的抗疲劳作用与急性毒性试验研究

Anti-fatigue effect and acute toxicity test of D-borneol refreshing atomization solution on mice

目的探讨不同剂量冰片提神雾化液对小鼠的抗疲劳作用及急性毒性,为其在临床上进一步开发和应用提供参考。方法将60只无特定病原体(specifc pathogen free,SPF)级昆明雄性小鼠分为空白组、4个剂量组,每组各12只小鼠,用于急性毒性实验和负重游泳实验。急性毒性实验,观察小鼠的不良反应,解剖后进行病理组织学检查。负重游泳实验,取小鼠血清、肝脏组织和后腿肌肉组织,测定抗疲劳生化指标如血清中葡萄糖(glucose,Glu)、乳酸(lactic acid,LD)、尿素氮(blood urea nitrogen,BUN)含量和乳酸脱氢酶(lactate dehydrogenase,LDH)水平;肝脏组织中肝糖原(liver glycogen,LG)含量;肌肉组织中肌糖原(muscle glycogen,MG)含量等;测定抗氧化指标如肌肉组织中丙二醛(malondialdehyde,MDA)含量、超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)水平等。结果在急性毒性实验中,冰片提神雾化液雾化期间各组小鼠精神状态、皮毛色泽及体质量均无异常,病理组织检查未见异常;负重游泳实验结果显示,中、高剂量冰片提神雾化液可有效延长小鼠负重力竭游泳时间(P<0.001),可显著提升游泳后小鼠MG含量(P<0.01),降低血清LD、LDH水平(P<0.05),且可降低血清BUN含量(P<0.01),升高血清中Glu水平(P<0.01),减少血清中MDA含量(P<0.05),提升SOD(P<0.01)和GSH-Px水平(P<0.001)。结论冰片提神雾化液无明显不良反应,具有良好的提神和抗疲劳效果,机制可能涉及提高抗氧化能力、减少肌肉损伤等途径。

Objective To investigate the anti-fatigue effects and acute toxicity of different doses of D-borneol refreshing atomization solution on mice,and to provide reference for the further development and application of D-borneol refreshing atomization solution in clinic.Methods Sixty specifc pathogen free(SPF)male KM mice were divided into one blank group and four dose groups,with 12 mice in each group,for acute toxicity experiments and weight-bearing swimming experiments.In the acute toxicity test,the toxic reaction of mice was observed.After dissection,histopathological examination was performed.For weight-bearing swimming experiment,serum,liver and hind leg muscle tissues of mice were collected.Blood glucose(Glu),lactic acid(LA),blood urea nitrogen(BUN),and lactate dehydrogenase(LDH)activity levels were measured in serum.Liver glycogen(LG)content was assessed in liver tissue;muscle glycogen(MG)and other anti-fatigue biochemical indicators were evaluated in muscle tissue.Malondialdehyde(MDA)content and the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were determined in muscle tissue.Results During the administration of D-borneol refreshing atomization solution,no abnormalities were observed in the mental state,fur color,or body weight of mice across all groups during the acute toxicity test.Pathological examinations did not reveal any abnormalities either.The weight-bearing swimming test results demonstrated that medium and high doses of D-borneol refreshing atomization solution could effectively prolong the time of weight-bearing exhaustive swimming in mice(P<0.001),significantly increase the content of MG(P<0.01),and reduce the activities of serum LD and LDH(P<0.05).The serum BUN level was decreased(P<0.01),the blood glucose level was increased(P<0.01),and the serum MDA(malondialdehyde)level was decreased(P<0.05).The levels of SOD(superoxide dismutase)(P<0.01)and GSH-Px(glutathione peroxidase)(P<0.001)were increased.Conclusion D-borneol refreshing atomization solution has no obvious toxic and side effects,and has good refreshing and anti-fatigue effects.The mechanism may be related to improving antioxidant capacity and reducing muscle damage.