病毒性脑炎患儿脑脊液IP-10、MCP-1检测的临床意义

Roles of IP-10 and MCP-1 in cerebrospinal fluid of children with viral encephalitis

目的探讨病毒性脑炎患儿脑脊液干扰素诱导蛋白-10(IP-10)和单核细胞趋化蛋白-1(MCP-1)检测的临床意义。方法选取2019年7月—2020年12月邢台市人民医院病毒性脑炎患儿60例(病毒性脑炎组),其中轻症26例、重症34例,以排除颅内感染的发热患儿40例作为对照组。采用酶联免疫吸附试验检测脑脊液IP-10、MCP-1和肿瘤坏死因子-α(TNF-α)水平。采用Pearson相关分析评估IP-10、MCP-1水平与TNF-α水平的相关性。结果病毒性脑炎组脑脊液IP-10、MCP-1和TNF-α水平均显著高于对照组(P<0.001)。重症组脑脊液IP-10、MCP-1和TNF-α水平均显著高于轻症组(P<0.05)。Pearson相关分析结果显示,病毒性脑炎组脑脊液IP-10、MCP-1水平与TNF-α水平均呈正相关(r值分别为0.742、0.696,P<0.05)。结论IP-10和MCP-1与儿童病毒性脑炎的病情严重程度有关,可作为病情监测指标。

Objective To investigate the roles of interferon-inducible protein-10(IP-10)and monocyte chemotactic protein-1(MCP-1)in the cerebrospinal fluid of children with viral encephalitis.Methods Totally,60 children with viral encephalitis(viral encephalitis group)were enrolled from Xingtai People's Hospital from July 2019 to December 2020,which included 26 mild cases and 34 severe cases,and 40 children with fever excluding intracranial infection were enrolled as control group.The levels of IP-10,MCP-1 and tumor necrosis factor-alpha(TNF-α)in cerebrospinal fluid were determined by enzyme-linked immunosorbent assay.The correlations of IP-10 and MCP-1 with TNF-α levels were evaluated by Pearson correlation analysis.Results The levels of cerebrospinal fluid IP-10,MCP-1 and TNF-α in viral encephalitis group were higher than those in control group(P<0.001).The levels of cerebrospinal fluid IP-10,MCP-1 and TNF-α in severe group were higher than those in mild group(P<0.05).The cerebrospinal fluid levels of IP-10 and MCP-1 were positively correlated with TNF-α levels in viral encephalitis group(r values were 0.742 and 0.696,P<0.05).Conclusions IP-10 and MCP-1 are correlated with the severity of viral encephalitis in children and can be used as one of the indicators of disease surveillance.