基于豚鼠模型结合网络药理学探究七白粉的皮肤美白作用机制

Exploring the Mechanism of Skin Whitening of Qibai Powder Based on the Guinea Pig Model Combined with Network Pharmacology

目的:研究七白粉的皮肤美白作用,并结合网络药理学和分子对接技术预测其达到美白效果的作用机制。方法:采用紫外线B波段(UVB)照射诱导豚鼠皮肤色素沉着,建立动物模型。观察七白粉对模型豚鼠的美白效果,同时检测黑色素细胞数和超氧化物歧化酶(SOD)、丙二醛(MDA)、酪氨酸酶(TYR)及脂褐质(LF)等与皮肤色素沉积相关的生化指标。利用网络药理学预测七白粉美白的作用机制,并通过分子对接和表面等离子体共振(SPR)进行验证。结果:在动物实验中,与模型组比较,七白粉可显著改善皮肤亮度,且黑色素细胞数和TYR、LF、MDA水平均显著降低,而SOD水平显著升高。网络药理学研究发现七白粉通过儿茶素没食子酸酯、茯苓酸C、别欧前胡素等关键成分,作用于蛋白激酶Bα(AKT1)、肿瘤坏死因子(TNF)、表皮生长因子(EGFR)等关键靶标,调控磷脂酰肌醇3-激酶-蛋白激酶B(PI3K-AKT)和TNF等信号通路改善黑色素沉积而发挥美白作用。分子对接和SPR结果显示关键成分与关键靶标有较好的亲和力。结论:七白粉可通过抗氧化和抑制TYR的活性达到美白皮肤的效果。此外,七白粉通过多成分作用于多靶点,调控多通路改善黑色素沉积而发挥美白作用,体现了中药复方君臣佐使相互协同,多成分,多靶点,多通路的特点。

Objective:To study the skin whitening effect of Qibai powder and predict its potential mechanism by combining network pharmacology and molecular docking technology.Methods:Ultraviolet B band(UVB)irradiation was used to induce skin pigmentation on the guinea pig to establish the animal model.The whitening effect of Qibai powder on model guinea pigs was observed,and the number of melanocytes and biochemical indicators related to skin pigmentation,such as medium superoxide dismutase(SOD),malondialdehyde(MDA),tyrosinase(TYR)and lipofuscin(LF),were also detected.The mechanism of whitening of Qibai powder was predicted using network pharmacology and validated by molecular docking and surface plasmon resonance(SPR).Results:In animal experiments,compared with the model group,Qibai powder significantly increased the brightness of the skin and reduced the melanocyte number,TYR,LF,and MDA levels,while significantly increasing SOD level.The network pharmacology study found that Qibai powder may act on key targets such as protein kinase Bα(AKT1),tumor necrosis factor(TNF),and epidermal growth factor(EGFR)through key components such as catechin gallate,pachymic acid C,and alloimperatorin and mainly regulate the PI3K-AKT and TNF signaling pathway to reduce melanin deposition to achieve the whitening effect.The molecular docking and SPR results showed that the key components have good affinities with the key targets.Conclusion:Qibai powder can achieve the skin whitening effect through antioxidants and inhibiting the activity of TYR.In addition,Qi Bai powder can reduce melanin deposition and exerts its whitening effects by acting on multi-targets through multi-ingredients and regulating multiple pathways.It reflects the synergistic effects of traditional Chinese medicines,as well as the characteristics of multi-components,multi-targets,and multi-pathways.