麻黄细辛附子汤对过敏性鼻炎小鼠神经通路的影响

Effect of Mahuang Xixin Fuzi Decoction on the Neural Pathway in Mice with Allergic Rhinitis

目的:探索麻黄细辛附子汤(MXFD)及其拆方对过敏性鼻炎(AR)的作用机制,明确其是否通过2型固有淋巴样细胞(ILC2s)上的神经通路神经调节肽U(NMU)/神经调节肽U受体1(NMUR1)发挥抗过敏作用。方法:C57BL/6J小鼠分别按单味药、两味药、三味药组合给药分为7组,使用鸡卵清蛋白(OVA)制备AR模型,造模成功后给予不同组合的中药灌胃治疗14 d。过敏反应行为学评分法评价模型是否成功;免疫荧光双染法检测肺组织中活化T细胞核因子2(NFAT2)的含量;逆转录实时定量聚合酶链式反应(RT-qPCR)检测肺组织中神经调节肽U受体1(NMUR1)的基因表达水平。结果:小鼠造模成功,各组NFAT2、NMUR1基因的表达水平均升高(均P<0.01)。中药治疗后,给药各组过敏反应行为学评分均降低(均P<0.01);NFAT2表达均降低(均P<0.01),麻黄组抑制效果最显著;除辛附组,其他组NMUR1的基因表达水平均降低(均P<0.01),麻附组、细辛组抑制效果最显著。结论:麻黄细辛附子汤可影响NMU/NMUR1通路抑制过敏性鼻炎,麻黄单用在抑制NFAT2环节效果最优,麻黄和附子合用、细辛单用抑制NMUR1表达效果最优。

Objective:To explore the mechanism of action of Mahuang Xixin Fuzi Decoction(MXFD)and its decomposed formula on allergic rhinitis(AR),to determine whether it exerts anti-allergic effects through the nerve pathway Neuromedin U(NMU)/Neuromedin U receptor 1(NMUR1)on type 2 innate lymphoid cells 2(ILC2s).Methods:C57BL/6J mice were divided into seven groups according to single,double,and triple herbal combinations.AR models were prepared using ovalbumin(OVA).After successful molding,mice in the treatment groups were treated with the different herbal combinations of MXFD for 14 days.The success of the model was evaluated by the allergic reaction behavioral scoring method;the content of nuclear nactor of activated T cells 2(NFAT2)in lung tissues was detected by immunofluorescence double-staining;and the expression level of NMUR1 gene in lung tissues was detected by reverse transcription real-time quantitative polymerase chain reaction(RT-qPCR).Results:The mice were successfully modeled,with increased expression levels of NFAT2 and NMUR1 genes in all groups(P<0.01).After treatment with Chinese medicine,the allergic reaction behavioral scores of the treated groups were reduced(P<0.01);the expression of NFAT2 was reduced(P<0.01),with the Mahuang group had the most significant inhibitory effect;except for the Xixin Fuzi group,the expression of NMUR1 gene was reduced(P<0.01)in all other groups,with the Mahuang Fuzi and Xixin groups had the most significant inhibitory effect.Conclusion:MXFD can inhibit allergic rhinitis by affecting the NMU/NMUR1 pathway.Mahuang alone has the best effect in inhibiting NFAT2,while the combination of Mahuang and Fuzi,Xixin alone have the best effect on NMUR1 expression.