基于SSR标记的148份玉米自交系亲缘关系分析

Genetic Relationship Analysis of 148 Maize Inbred Lines Based on SSR Molecular Markers

利用40对核心SSR标记分析148份玉米自交系的遗传多样性与亲缘关系。结果表明,40对核心SSR标记在148份玉米自交系中共检测出136个等位变异,每个SSR标记得出的等位变异总数为2~6个,平均3.4个;有效等位基因数(Ne)变幅为1.2323~5.0050,平均2.3939;基因多样性变幅为0.1885~0.8002,平均为0.5252;引物的多态性信息含量(PIC)变幅为0.1781~0.7705,平均0.4617。利用UPGMA聚类法将148份玉米自交系划分为Reid、旅大红骨、PB、Lancaster、塘四平头、中间类群,共6个类群,其中,主要以Reid、旅大红骨、PB、Lancaster和塘四平头这5个类群为主,种群的划分与系谱基本吻合。

Forty core SSR markers were used to explore the genetic diversity and genetic relationship of 148 maize inbred lines.The results indicated that,a total of 136 allelic variations were detected across the 148 maize inbred lines using the 40 core SSR markers,with each marker exhibiting 2 to 6 alleles and an average of 3.4 alleles.The effective number of alleles(Ne)was 1.2323 to 5.0050,with an average of 2.3939.The gene diversity ranged from 0.1885 to 0.8002,with an average of 0.5252.The polymorphic information content(PIC)of SSR markers was between 0.1781 and 0.7705,with an average of 0.4617.By UPGMA cluster analysis method,148 maize inbred lines were divided into 6 major groups,which were named Reid,Luda Red Cob,PB,Lancaster,Sipingtou,and uncertain group.The division of populations largely aligned with the 5 principal groups of Reid,Luda Red Cob,PB,Lancaster,and Sipingtou,reflecting a fundamental agreement with the genealogy.