改良DSS法制作稳定泡沫的效果评价

A modified DSS method producing more stable sclerosant foam

目的:对双注射器系统(double-syringe system,DSS)进行改良,使之能够制作出更稳定的硬化剂泡沫。方法:分别用经典DSS方法和改良DSS方法制备1%聚多卡醇(polidocanol,POL)泡沫。在改良DSS方法中,用自制弹性气囊替代DSS方法中的一个注射器。改良DSS方法制备POL泡沫过程如下,先用注射器抽取2 mL POL液体,再抽取8 mL空气;之后将注射器与连有自制气囊的三通直线连接,反复推拉注射器20次,产生泡沫。采用泡沫半衰期(foam half-time,FHT)和显微镜观察,比较2种泡沫稳定性及均一性。检测改良DSS方法制备中泡沫体积的变化,比较扭转三通角度后,改良DSS方法制备泡沫的质量。采用GraphPad Prism 8.0软件包对于数据进行统计学分析。结果:用自制弹性气囊替代经典DSS方法中单个注射器的改良DSS方法,能够制备出半衰期更长、微泡更均一的泡沫,可能与泡沫制备过程中的体积压缩有关。同时,改良DSS方法可以通过扭转三通角度,进一步提升泡沫质量。结论:改良DSS方法用弹性气囊替代经典DSS方法中的单个注射器,能够产生更稳定的泡沫。

PURPOSE:To modify the classical double-syringe system(DSS)method and to make it capable of producing more stable foam.METHODS:1%polidocanol(POL)foam was generated by DSS method and modified DSS method,respectively.In modified DSS method,one syringe was replaced by a self-made elastic gasbag.The procedure for modified DSS was as follows:drew 2 mL POL liquid and 8 mL air into the syringe;connected the syringe to a three-way tap with a fixed gasbag in a straight line;pushed the syringe plunger 20 times.The stability and uniformity of foam produced with two methods were compared using foam half-time(FHT)and optical microscopy,respectively.The change of foam volume in modified DSS method was also detected.The quality of foam produced by modified DSS method whether three-way tap was twisted was compared.GraphPad Prism 8.0 software package was used for data analysis.RESULTS:Replacing one syringe in the DSS method with an elastic gasbag could produce more uniform foam with longer FHT,which maybe related to foam volume compression.At the same time,the foam quality could be further improved by twisting the three-way tap in modified DSS method.CONCLUSIONS:Replacing one syringe in the DSS method with an elastic gasbag could produce more stable foam.