结肠癌病人血清miR-448和miR-153表达对肠道菌群及免疫功能的影响

The impacts of serum miR-448 and miR-153 expression on intestinal microbiota and immune function in colon cancer patients

目的探讨结肠癌病人血清微小RNA-448(miR-448)和微小RNA-153(miR-153)表达对肠道菌群及免疫功能的影响。方法2020年2月~2023年2月期间收治的127例结肠癌病人作为研究组,89例结肠良性病变病人作为结肠良性病变组,同期本院体检的127例健康者作为对照组。以血清miR-448(1.12)和miR-153(0.98)表达水平的中位数为临界值将研究组病人分为miR-448高表达组(63例)、miR-448低表达组(64例),miR-153高表达组(64例)、miR-153低表达组(63例)。Pearson法分析血清miR-448和miR-153表达水平与结肠癌病人肠道菌群、免疫功能指标的相关性;受试者工作特征(ROC)曲线分析血清miR-448和miR-153对结肠癌的诊断价值。结果研究组、结肠良性病变组、对照组大肠杆菌、双歧杆菌、双歧杆菌/大肠杆菌比值以及免疫球蛋白IgG、IgM、IgA比较,差异有统计学意义(P<0.05);研究组血清miR-448表达水平为(1.25±0.22),高于对照组的(1.09±0.03)和结肠良性病变组的(1.18±0.15),血清miR-153表达水平为(0.89±0.12)低于对照组的(1.03±0.17)和结肠良性病变组的(0.96±0.16);血清miR-448、miR-153高表达和低表达组大肠杆菌、双歧杆菌、双歧杆菌/大肠杆菌比值、IgG、IgM、IgA表达水平比较,差异有统计学意义(P<0.05);血清miR-448、miR-153与大肠杆菌、双歧杆菌、双歧杆菌/大肠杆菌比值有相关性(r=0.657,-0.742,-0.753;r=-0.711,0.813,0.771,P<0.05);IgG、IgM、IgA与血清miR-448呈正相关(r=0.686,0.709,0.744),与miR-153呈负相关(r=-0.690,-0.841,-0.769,P<0.05);血清miR-448和miR-153联合检测诊断结肠癌的AUC为0.845,优于各自单独检测(Z二者联合-miR-448=4.561、Z二者联合-miR-153=2.426,P=0.000、0.015)。结论结肠癌病人血清miR-448和miR-153表达水平与肠道菌群及免疫功能有相关性,二者联合对结肠癌具有较高的诊断价值。

Objective To investigate the impacts of serum microRNA-448(miR-448)and microRNA-153(miR-153)expression on intestinal microbiota and immune function in colon cancer patients.Methods A total of 127 patients with colon cancer admitted to our hospital from February 2020 to February 2023 were collected as the study group,89 patients with benign colon lesions were collected as the benign colon lesion group,and 127 healthy individuals who underwent physical examination in our hospital during the same period were collected as the control group.The study group were grouped into miR-448 high expression group(63 cases)and miR-448 low expression group(64 cases),and the miR-153 high expression group(64 cases)and miR-153 low expression group(63 cases)based on the median expression levels of serum miR-448(1.12)and miR-153(0.98)as the critical values.Pearson method was applied to analyze the correlation between the expression levels of serum miR-448 and miR-153 and intestinal microbiota and immune function indicators in colon cancer patients;receiver operating characteristic(ROC)curve was plotted to analyze the diagnostic value of serum miR-448 and miR-153 for colon cancer.Results There were significant differences among the study group,the benign colon disease group and the control group in terms of Escherichia coli,bifidobacteria,bifidobacteria/Escherichia coli ratio,and immunoglobulin(Ig)G,IgM,IgA(P<0.05);The expression level of serum miR-448 in the study group(1.25±0.22)was higher than that in the control group(1.09±0.03)and colon benign lesion group(1.18±0.15),while the expression level of serum miR-153(0.89±0.12)was lower than that in the control group(1.03±0.17)and colon benign lesion group(0.96±0.16)(P<0.05);there were significant differences in the Escherichia coli,bifidobacteria,bifidobacteria/Escherichia coli ratio,the expression levels of IgG,IgM,and IgA between the high and low expression groups of serum miR-448 and miR-153(P<0.05).There was significant correlation between serum miR-448,miR-153 and Escherichia coli,bifidobacteria,bifidobacteria/Escherichia coli ratio(r=0.657,-0.742,-0.753;r=-0.711,0.813,0.771,P<0.05);IgG,IgM,IgA were positively correlated with serum miR-448(r=0.686,0.709,0.744),and negatively correlated with miR-153(r=-0.690,-0.841,-0.769,P<0.05);the combined detection of serum miR-448 and miR-153 for the diagnosis of colon cancer had an AUC of^0.845,which was better than the individual detection(Z combination-miR-448=4.561,Z combination-miR-153=2.426,P=0.000、0.015).Conclusion The expression levels of serum miR-448 and miR-153 in patients with colon cancer are correlated with intestinal microbiota and immune function,and the combination of the two has high diagnostic value for colon cancer.