基于双发射碳量子点的比率型荧光探针检测阿司匹林

Ratiometric fluorescent sensor based on double emission carbon quantum dots for the detection of Aspirin

以间苯二胺和酒石酸为原料,通过一步水热法合成了在单一激发波长下具有双发射特性的碳量子点(CQDs)。利用透射电镜(TEM)、傅里叶变换红外光谱(FTIR)、X射线光电子能谱(XPS)、紫外-可见吸收光谱(UV-vis)和荧光光谱对其结构和光学性质进行了表征。当激发波长为390 nm时,该CQDs在440和502 nm处有2个发射峰。阿司匹林能够使502 nm处的发射峰荧光强度增加,而440 nm处的发射峰强度基本不变,基于此,构建了一种CQDs比率型荧光探针检测阿司匹林的新方法。阿司匹林浓度在0.5~160μmol/L范围内与CQDs的2个发射峰的荧光强度比(F_(502)/F_(440))呈良好的线性关系,检出限低至0.062μmol/L。该方法成功用于药品中阿司匹林的检测。

Carbon quantum dots(CQDs)with dual emission property at a single excitation wavelength were synthesized by a one-step hydrothermal method using m-phenylenediamine and tartaric acid as raw materials.The structure and optical properties of CQDs were characterized by transmission electron microscope,Fourier transform infrared spectrometer,X-ray photoelectron spectroscopy,ultraviolet-visible absorption spectroscopy and fluorescence spectroscopy.When the excitation wavelength was 390 nm,the CQDs had two emission peaks at 440 and 502 nm.The presence of aspirin can increase the fluorescence intensity of the emission peak at 502 nm,while the intensity of the emission peak at 440 nm was basically unchanged.Based on this,a novel CQDs based ratiometric fluorescent sensor was constructed for the determination of aspirin.The concentration of aspirin in the range of 0.5-160μmol/L had a good linear relationship with the fluorescence intensity ratio(F_(502)/F_(400))of the two emission peaks of CQDs,and the detection limit was as low as 0.062μmol/L.The developed method has been successfully applied to the determination of aspirin in actual drugs.