9种中药益生菌复方抗病毒性炎症功效的比较

Comparison of the anti-viral-inflammatory of 9 kinds of traditional Chinese medicine probiotics

目的 了解9种中药益生菌复方抗病毒性炎症的能力。方法 MTT实验评估9种中药益生菌复方的生物安全性;利用聚基胞苷酸[poly(I:C)]诱导巨噬细胞RAW 264.7发生炎症反应,检测细胞上清液中白细胞介素6(IL-6)水平。Poly(I:C)诱导绿色荧光蛋白标记中性粒细胞的斑马鱼鱼鳔发生炎症反应,统计鱼鳔处中性粒细胞水平。结果 9种中药益生菌复方中,复方8和9号对poly(I:C)诱导RAW264.7细胞发生炎症反应表现出剂量依赖性的抗炎功效,复方4和7号仅在高剂量时可抑制细胞炎症反应(P<0.01或0.05)。高剂量6、8号复方及中高剂量9号复方斑马鱼鱼鳔处中性粒细胞个数均少于模型对照组(P<0.01或0.05)。结论 9种中药益生菌复方中9号复方的抗炎效果最好,对poly(I:C)诱导RAW 264.7细胞释放炎症因子IL-6抑制率最高,并且在poly(I:C)诱导的斑马鱼炎症模型中能显著阻断中性粒细胞向鱼鳔处迁徙,具有较高的抗病毒性炎症功效。

Objective To compare the anti-viral-inflammatory ability of 9 kinds of traditional Chinese medicine probiotics,and select the most effective anti-viral-inflammatory traditional Chinese medicine probiotics for the treatment of viral-inflammation.Methods The cytotoxicity and biocompatibility of 9 kinds of traditional Chinese medicine probiotics were compared by using MTT methods.Poly(I:C)was used to induce macrophage RAW 264.7 to initiate an inflammatory response.The concentration of inflammatory marker interleukin-6(IL-6)in the cell supernatant was detected to evaluate the anti-inflammatory efficacy of the traditional Chinese medicine probiotics at the cellular level.In animal experiments,9 kinds of traditional Chinese medicine probiotics were pretreated,and then poly(I:C)was injected into zebrafish labeled with green fluorescent protein neutrophils as inducer,and the inflammation model of zebrafish swim bladder was constructed.The number of neutrophils in the swim bladder was observed and counted by fluorescence microscope.Results Among the 9 kinds of Chinese herbal probiotic compounds,Compound 8 and 9 showed dose-dependent anti-inflammatory effect on RAW264.7 cells induced by poly(I:C),while compound 4 and 7 inhibited cell inflammatory response only at high doses(P<0.01 or 0.05).The number of neutrophils in the swim bladder of zebrafish in high-dose compound 6 and 8 and medium-high-dose compound 9 were lower than those in model control group(P<0.01 or 0.05).Conclusion Among the 9 kinds of traditional Chinese medicine probiotics,No.9 had the highest efficiency for anti-inflammatory,and the highest inhibitory rate on the release of inflammatory factor IL-6 from RAW 264.7 cells induced by poly(I:C),and significantly blocked the migration of neutrophils to the swim bladder in zebrafish inflammation mode,showing a highly effective anti-inflammatory effect.