子宫肌瘤患者血清miR-29和miR-210表达水平变化及临床意义

The expression changes and clinical significance of serum miR⁃29 and miR⁃210 in patients with uterine fibroids

目的检测子宫肌瘤患者血清微小RNA-29(miR-29)和miR-210表达水平变化,并分析其临床意义。方法选取2016年6月-2019年6月首都医科大学附属北京潞河医院收治的143例子宫肌瘤患者作为研究组,另取同期体检且一般资料与研究组相匹配的100名健康者作为对照组。采用Pearson法分析子宫肌瘤患者血清miR-29和miR-210表达水平的相关性,多因素logistic回归分析影响子宫肌瘤发生的相关因素,受试者工作特征(ROC)曲线分析血清miR-29和miR-210对子宫肌瘤的诊断价值。结果研究组血清miR-29(1.33±0.36)和miR-210(1.26±0.33)表达水平均显著高于对照组miR-29(1.02±0.16)和miR-210(1.07±0.19),差异均有统计学意义(t=8.068、5.186,P均<0.001)。子宫肌瘤患者血清miR-29与miR-210表达水平成正相关(r=0.784,P<0.05)。研究组有盆腔炎史、腹壁脂肪厚度≥20 mm、乳腺增生患者所占比例高于对照组,差异均有统计学意义(χ^(2)=4.879、4.559、4.622,P均<0.05)。血清miR-29和miR-210单独检测诊断子宫肌瘤的曲线下面积(AUC)分别为0.762、0.802,二者联合检测的AUC为0.880,联合检测优于各自单独检测(Z_(联合vs.miR-29)=5.817,P<0.001;Z_(联合vs.miR-210)=2.928,P=0.003)。有盆腔炎史、腹壁脂肪厚度≥20 mm、有乳腺增生、miR-29≥1.11、miR-210≥1.17均为发生子宫肌瘤的危险因素(P均<0.05)。结论血清miR-29和miR-210表达水平与子宫肌瘤的发生密切相关,二者联合检测对子宫肌瘤具有较高的诊断价值。

Objective To investigate the expression changes and clinical significance of serum microRNA⁃29(miR⁃29)and miR⁃210 in patients with uterine fibroids.Methods A total of 143 patients with uterine fibroids admitted to a tertiary hospital in Beijing from June 2016 to June 2019 were regarded as the study group,and 100 healthy individuals who underwent physical examination in this hospital during the same period and matched the general information with patients in the study group were collected as the control group.Pearson method was applied to analyze the correlation between serum miR⁃29 and miR⁃210 expression levels in patients with uterine fibroids;multivariate logistic regression was applied to analyze the relevant factors affecting the occurrence of uterine fibroids;receiver operating characteristic(ROC)curve was applied to analyze the diagnostic value of serum miR⁃29 and miR⁃210 for uterine fibroids.Results The expression levels of serum miR⁃29(1.33±0.36)and miR⁃210(1.26±0.33)in the study group were significantly higher than those in the control group(1.02±0.16),(1.07±0.19),the differences were statistically significant(t=8.068,5.186;all P<0.001).The expression levels of serum miR⁃29 and miR⁃210 in patients with uterine fibroids were positively correlated(r=0.784,P<0.05).The proportions of patients with a history of pelvic inflammatory disease,abdominal wall fat thickness≥20 mm,and breast hyperplasia in the study group were higher than those in the control group,the differences were statistically significant(χ^(2)=4.879,4.559,4.622;all P<0.05).The area under the curve(AUC)of serum miR⁃29 and miR⁃210 for diagnosing uterine fibroids was 0.762 and 0.802,respectively;the AUC for the combined detection of the two was 0.880;the combined detection of the two was better than that of serum miR⁃29 and miR⁃210 alone(Z_(combined vs.miR⁃29)=5.817,P<0.001;Z_(combined vs.miR⁃210)=2.928,P=0.003).History of pelvic inflammation,abdominal wall fat thickness≥20 mm,breast hyperplasia,miR⁃29≥1.11,miR⁃210≥1.17 were all risk factors for uterine fibroid(P<0.05).Conclusion Serum miR⁃29 and miR⁃210 were closely related to the occurrence of uterine fibroids,and their combined detection had high diagnostic value for uterine fibroids.