不同株型渠首1号上部烟叶代谢图谱差异分析

Difference Analysis of Metabolic Profiles of Upper Leaves from Different Plant Types of Qushou No.1

为明确烤烟不同株型产质量及代谢组学的差异,以渠首1号为研究对象,利用液相色谱-质谱联用(LC-MS)非靶向代谢组学方法并结合化学成分及感官质量等指标的测定,对筒形和伞形2种株型成熟时期上部烟叶代谢物进行分析。结果表明,与伞形烟株相比,筒形烟株株高和有效叶片数增加、最大叶面积减小。筒形烟株烟叶的整体化学成分协调性和感官质量均优于伞形烟株。筒形烟株产量较伞形烟株降低5.2%,但均价和产值分别增加25.0%和20.4%。通过偏最小二乘法判别分析法(PLS-DA)筛选出123种差异代谢物,主要是氨基酸、有机酸、糖类、类黄酮和生物碱类代谢物,其中N-甲酰-L-蛋氨酸、2-氧基精氨酸、苯乙酰谷氨酰胺、海藻糖、3-酮-β-D-半乳糖、D-甘露糖、2-苯乙醇、茉莉酸等可能是响应不同株型的关键代谢物。差异代谢物显著富集在氨基酸代谢、糖代谢等通路上,其中烟酸和烟酰胺代谢通路,丙氨酸、天冬氨酸和谷氨酸代谢通路,色氨酸代谢通路等在不同株型烟叶内在质量形成过程中起重要调控作用。

In order to clarify the differences in yield,quality and metabolomics of flue⁃cured tobacco under different plant types,with Qushou No.1 as the experimental material,the metabolites of upper leaves at the mature stage of two plant types were analyzed by LC⁃MS non⁃targeted metabonomics technique combined with the determination of chemical components and sensory quality.The results showed that compared with umbrella⁃type tobacco plants,the plant height and effective leaf number of cylinder⁃type tobacco plants increased,and the maximum leaf area decreased.The overall chemical composition coordination and sensory quality of the cylinder⁃type tobacco plants were better than those of the umbrella⁃type tobacco plants.The yield of cylinder⁃type tobacco plants was 5.2%lower than that of umbrella⁃type tobacco plants,but the average price and output value increased by 25.0%and 20.4%.Through partial least squares⁃discriminate analysis(PLS⁃DA),123 differential metabolites were screened out,including amino acids,organic acids,sugars,flavonoids and alkaloids,in which N⁃formyl⁃L⁃methionine,2⁃oxoarginine,phenylacetylglutamine,trehalose,3⁃keto⁃β⁃D⁃galactose,D⁃mannose,2⁃phenylethanol,jasmonic acid might be the key metabolites in response to different plant types.The differential metabolites were significantly enriched in the pathways of amino acid metabolism and glucose metabolism.Among them,nicotinic acid and nicotinamide metabolism pathway,alanine,aspartic acid and glutamic acid metabolism pathway,and tryptophan metabolism pathway played important regulating roles in the formation of intrinsic quality under different plant types.