山柰酚抗运动性疲劳的作用研究

Effects of Kaempferol against exercise fatigue

目的探讨山柰酚(Kae)的抗运动性疲劳作用及其作用机制。方法将大鼠分成对照组、模型组和低、中、高剂量实验组,每组12只。低、中、高剂量实验组分别灌胃给予50、100和200 mg·kg^(-1)的山柰酚溶液;模型组和对照组均灌胃给予等量的5%羧甲基纤维素钠。5组大鼠每天给药1次,连续给药4周。模型组和低、中、高剂量实验组大鼠在第2~4周进行力竭游泳运动。用试剂盒检测肝糖原含量、血清乳酸(LA)水平和腓肠肌组织超氧化物歧化酶(SOD)水平,用蛋白质印迹法检测腓肠肌组织中磷酸化-AMP依赖的蛋白激酶α(p-AMPKα)和过氧化物酶体增殖物激活受体γ共激活因子α(PGC-1α)的表达水平。结果对照组、模型组和中、高剂量实验组的肝糖原含量分别为(11.22±0.71)、(2.96±0.20)、(5.20±0.64)和(6.06±0.42)mg·g^(-1),血清LA水平分别为(0.31±0.03)、(0.90±0.03)、(0.50±0.03)和(0.40±0.03)mg·L^(-1),腓肠肌SOD水平分别为(20.12±0.80)、(30.02±2.70)、(40.04±2.31)和(45.56±1.77)U·mg prot^(-1),p-AMPKα/AMPKα比值分别为0.60±0.03、0.19±0.02、0.45±0.11和0.49±0.05,PGC-1α蛋白相对表达水平分别为0.80±0.03、0.10±0.01、0.49±0.10和0.53±0.11。对照组和中、高剂量实验组的上述指标与模型组比较,在统计学上差异均有统计学意义(均P<0.05)。结论山柰酚可提高运动性疲劳大鼠的抗疲劳能力,改善能量代谢,减少有害代谢物的积累,提高抗氧化活性,激活AMPK/PGC-1α信号通路。

Objective To investigate the anti-exercise fatigue effects and mechanism of Kaempferol(Kae).Methods The rats were randomly divided into control group,model group and experimental-L,-M,-H groups with 12 cases per group.Experimental-L,-M,-H groups were given 50,100 and 200 mg·kg^(-1)Kaempferol,respectively.Model and control groups were given equal amounts of 5%carboxymethylcellulose sodium by intragastric administration.Five groups were treated for 4 weeks with once a day.Rats in model group and experimental-L,-M,-H groups were given exhaustive swimming exercise at the 2nd-4th week.The contents of liver glycogen,serum lactate(LA)levels and superoxide dismutase(SOD)levels of gastrocnemius were measured according to the kit.The expression levels of phosphorylated-AMP dependent protein kinaseα(p-AMPKα)in gastrocnemius was detected by Western blot.Results The contents of liver glycogen in control group,model group and experimental-M,-H groups were(11.22±0.71),(2.96±0.20),(5.20±0.64)and(6.06±0.42)mg·g^(-1);the serum LA levels were(0.31±0.03),(0.90±0.03),(0.50±0.03)and(0.40±0.03)mg·L^(-1);the SOD levels in gastrocnemius muscle were(20.12±0.80),(30.02±2.70),(40.04±2.31)and(45.56±1.77)U·mg prot-1;the relative expression levels of p-AMPKα/AMPKαwere 0.60±0.03,0.19±0.02,0.45±0.11 and 0.49±0.05;the relative expression levels of PGC-1αwere 0.80±0.03,0.10±0.01,0.49±0.10 and 0.53±0.11,respectively.Compared with the model group,the above indexes of control group and experimental-M,-H groups were statistical significance(allP<0.05).Conclusion Kaempferol can improve the anti-fatigue ability of exercise-induced fatigue rats,improve energy metabolism,reduce accumulation of harmful metabolites,increase antioxidant activity,and activate AMPK/PGC-1αsignaling pathway.