ODF1在胃肠道神经内分泌肿瘤中的表达及临床意义

Expression of ODF1 in gastrointestinal tract neuroendocrine neoplasms and its clinical significance

目的探讨ODF1在胃肠道神经内分泌肿瘤(NEN)中的表达及与临床病理特征和预后的关系。方法收集2008-01—2021-12;胃肠道NEN标本254例,包括168例神经内分泌瘤(NETs)和86例神经内分泌癌(NEC)。采用免疫组化法检测ODF1在上述组织中的表达,分析其与临床病理特征和预后的关系。结果ODF1在168例NETs中全部弥漫阳性表达,在86例NEC中的阳性表达率为24.4%,而且均为局灶阳性染色。ODF1在胃肠道NETs和NEC中的表达差异有统计学意义(P<0.05)。ODF1的表达随着NEN分级增高、T分期增加、淋巴结转移和TNM分期增加而降低(P<0.05)。ODF1的表达与患者性别和年龄无关(P>0.05)。Kaplan-Meier生存分析和单因素COX分析显示ODF1阳性NEC患者的预后较好,多因素COX分析显示ODF1表达不是NEC独立的预后因子。结论ODF1在胃肠道NETs中表达显著高于NEC。ODF1水平随着NEN分级增高、T分期增加、淋巴结转移和TNM分期增加而降低,对预后预测有一定价值。

Objective To investigate the expression of outer dense fiber of sperm tails 1(ODF1) in gastrointestinal tract neuroendocrine neoplasms(NEN) and its relationship with clinicopathological features and prognosis. Methods A total of 254 NEN samples, including 168 neuroendocrine tumours(NET) and 86 neuroendocrine carcinomas(NEC) were collected from January 2008 to December 2021, ODF1 expression in the above tissues was detected by immunohistochemistry. The relationship between their expression and clinicopathological features and prognosis was analyzed. Results Immunohistochemical staining showed that the expression of ODF1 were all diffuse positive in 168 NETs samples, but the positive rate of ODF1 in 86 NEC was 24.4% and the staining pattern were focally positive. The expression difference of ODF1 in gastrointestinal NET and NEC was statistically significant(P<0.05).The expression of ODF1 decreased with the increase of NEN grade, T stage, lymph node metastasis, and TNM stage(P<0.05).The expression of ODF1 was not related to gender and age(P>0.05). Kaplan-Meier and univariate analysis showed that the expression of ODF1 was related to better prognosis in patients with NEC(P<0.05). Cox multivariate analysis showed that ODF1 expression was not the independent factor in patients with NEC. Conclusion ODF1 is highly expressed in gastrointestinal NETs and less so in NEC.ODF1 expression decreases with higher grading of NEN, increased T-stage, lymph node metastasis and TNM stage, with low expression suggesting poor prognosis.