苦荞NRAMP家族全基因组鉴定

Genome-Wide Identification of the NRAMP Gene Family in Fagopyrum tataricum

天然抗性相关巨噬细胞蛋白(Natural Resistance Associated Macrophage Protein,NRAMP)是一个高度保守的膜蛋白家族,在植物体中广泛参与过渡金属离子的跨膜转运。本研究基于基因组学,从重要粮食作物苦荞(Fagopyrum tataricum)的基因组中鉴定出11个NRAMP基因,并通过TBtools、Cell-PLoc、MEGA、SWISS-MODEL、SOPMA等工具对该家族成员的理化性质、保守基序、基因结构、系统发育关系、三维结构、顺式作用元件等方面进行了系统分析。结果发现,苦荞NRAMP基因家族在相同亚族下的保守基序和基因结构高度一致。此外,苦荞NRAMP基因的启动子区域中含有大量植物激素响应元件及胁迫响应元件,亚族间的元件分布存在明显差异。对苦荞NRAMP基因编码蛋白的三维结构、亚细胞定位以及顺式作用元件分析发现,FtNRAMP4、FtNRAMP10、FtNRAMP11三个成员和其他FtNRAMP具有差异。苦荞NRAMP蛋白的二级结构以α-螺旋为主,占比为35.37%~63.00%。本研究结果为深入挖掘苦荞NRAMP基因家族功能提供了科学依据。

Natural Resistance Associated Macrophage Protein(NRAMP),a highly conserved membrane protein family,plays a widespread role in the transmembrane transport of transition metal ions in plants.In this study,based on genomics,we identify 11 NRAMP genes from the genome of the important cereal crop Fagopyrum tataricum.A systematic analysis of the physical and chemical properties,conserved motifs,gene structures,phylogenetic relationships,three-dimensional structures,and cis-acting elements of the family members is conducted by TBtools,Cell-PLoc,MEGA,SWISS-MODEL,SOPMA and other tools.The results reveal a high consistency in conserved motifs and gene structures within the NRAMP gene family in Fagopyrum tataricum under the same subfamily.Additionally,the promoter regions of Fagopyrum tataricum NRAMP genes contained numerous plant hormone response elements and stress response elements,with distinct distribution patterns among subfamilies.Analysis of the three-dimensional structures,subcellular localization,and cis-acting elements of the encoded proteins of Fagopyrum tataricum NRAMP genes identify differences,particularly in FtNRAMP4,FtNRAMP10,and FtNRAMP11 compared to other FtNRAMP members.The secondary structure of Fagopyrum tataricum NRAMP members is predominantly composed of α-helices,ranging from 35.37%to 63.00%.The results of this study provide a scientific basis for further exploring the functions of the Fagopyrum tataricum NRAMP gene family.