胡麻籽饼粕黄酮的降脂降糖效果的研究

Study on the Effect of Flavonoids from Flaxseed Meal on Reducing Lipid and Glucose

目的:探究胡麻籽饼粕黄酮对高脂膳食小鼠体内血糖血脂代谢异常的影响。方法:体外实验部分,分析胡麻籽饼粕黄酮对超氧自由基的清除作用、对α-葡萄糖苷酶活性和α-淀粉酶活性抑制作用;动物实验部分,将40只昆明小鼠随机均分为4组,正常对照组(CON)、高脂膳食组(HF)、低剂量黄酮组(LH)和高剂量黄酮组(HH);持续饲喂5周后,分析小鼠血糖、血脂、抗氧化酶水平的变化。结果:胡麻籽饼粕黄酮对超氧自由基有较强的清除效果,对α-葡萄糖苷酶活性和α-淀粉酶活性有较好的抑制效果;高脂膳食饲喂导致小鼠体内血糖、血脂水平的异常,降低抗氧化酶活力;胡麻籽饼粕黄酮能够显著提高小鼠体内抗氧化酶活力,并显著改善血脂水平和血糖浓度异常。结论:胡麻籽饼粕黄酮具有改善高脂膳食导致的小鼠机体血糖血脂代谢异常的作用,降低机体氧化应激。

Objective:To investigate the effect of flavonoids from flaxseed meal on abnormal metabolism of blood glucose and lipid in mice fed with high fat diet.Methods:In vitro experimental part,the scavenging effects of flavonoids from flaxseed meal on superoxide radicals,and their inhibitory effects on α-glucosidase and α-amylase activities are analyzed;animal experiment part,40 Kunming mice are randomly and evenly divided into 4 groups,normal control group(CON),high-fat diet group(HF),low-dose flavonoid group(LH)and high-dose flavonoid group(HH);after 5 weeks of continuous feeding,the levels of blood glucose,blood lipid and antioxidant enzyme are analyzed.Results:Flavonoids from flaxseed meal could scavenge superoxide radical,inhibit α-glucosidase activity and α-amylase activity;highfat diet could abnormalities the level of blood glucose and blood lipid and decrease the activity of antioxidant enzymes in mice;flavonoids from flaxseed meal could significantly increase the activity of antioxidant enzymes in mice,and significantly improve the abnormal level of blood lipids and blood glucose concentration.Conclusion:Flavonoids from flaxseed meal has the effect of intervening the abnormal metabolism of blood glucose and lipid in mice caused by highfat diet and reducing oxidative stress.