荔枝草中乙酸乙酯层化学成分分析

Analysis of the Chemical Composition of Ethyl Acetate Layer in Salvia plebeia

目的:对荔枝草的乙酸乙酯层进行化学成分研究。方法:采用硅胶柱色谱、中压快速色谱和Sephadex-LH凝胶色谱等方法进行分离纯化,运用波谱手段、理化性质和文献对照进行各单体化合物的结构鉴定。结果:从荔枝草的乙酸乙酯层分离出2个单体化合物,分别鉴定为泽兰黄酮和高车前素。结论:采用中压色谱能从荔枝草乙酸乙酯萃取层中快速大量提取泽兰黄酮和高车前素,该方法省时、简便、高效,值得推广应用。

Objective:To study the chemical composition of ethyl acetate layer in Salvia plebeia.Methods:Silica gel column chromatography,medium-pressure rapid chromatography and Sephadex-LH gel chromatography are used for separation and purification,and the structure of each monomer compound is identified by spectroscopy,physicochemical properties and literature control.Results:Two monomeric compounds are isolated from the ethyl acetate layer of Salvia plebeia,which are identified as zealand flavonoids and homopsyllin,respectively.Conclusion:For the first time,medium-pressure chromatography is used to quickly obtain zealand flavonoids and homopsyllin from the ethyl acetate extraction layer of Salvia plebeia,which is time-saving,simple and efficient,and worthy of popularization and application.