基于5-HT-BDNF轴研究针刺联合柴胡疏肝方对大鼠缺血再灌注脑损伤的保护作用及机制

Protective effect and mechanism of acupuncture combined with Chaihu Shugan Recipe on cerebral ischemia reperfusion injury in rats based on 5-HT-BDNF axis study

目的探讨针刺联合柴胡疏肝方对脑卒中大鼠脑功能的保护活动和机制。方法构建大鼠大脑中动脉闭塞(MCAO)模型,针刺联合柴胡疏肝方治疗前后进行脑功能评价;酶联免疫吸附试验检测各组大鼠脑组织中单胺类神经递质5-羟色胺(5-HT)、多巴胺(DA)和去甲肾上腺素(NE)的表达水平;免疫印迹法检测各组大鼠脑组织中脑源性神经营养因子(BDNF)蛋白表达水平。结果相比于针刺或柴胡疏肝方单独治疗,针刺联合柴胡疏肝方对大鼠脑卒中模型的脑功能损伤具有更好的保护作用;针刺联合柴胡疏肝方能够上调大鼠脑组织中单胺类递质5-HT、DA和NE的表达;针刺联合柴胡疏肝方能够上调大鼠脑组织中BDNF蛋白表达。结论针刺联合柴胡疏肝方对大鼠缺血再灌注脑损伤具有更好的保护作用,其可能与单胺类神经递质的相互作用以及上调BDNF蛋白的表达有关。

Objective To explore the protective activity and mechanism of acupuncture combined with Chaihu Shugan Recipe on brain function of stroke rats.Methods The middle cerebral artery occlusion(MCAO)model was establis hed in rats,and the brain function was evaluated before and after acupuncture combined with Chaihu Shugan Recipe.Enzyme linked immunosorbent assay(ELISA)was used to detect the expression levels of monoamine neurotransmitters 5-hydroxytryptamine(5-HT),dopamine(DA),and norepinephrine(NE)in the brain tissues of rats in each group.Western blotting was used to detect the expression level of brain-derived neurotrophic factor(BDNF)protein in the brain tissue of rats in each group.Results Compared with acupuncture or Chaihu Shugan Recipe alone,acupuncture combined with Chaihu Shugan Recipe had a better protective effect on brain function injury in rat stroke model.Acupuncture combined with Chaihu Shugan Recipe can up-regulate the expression of 5-HT,DA and NE in brain tissue of rats.Acupuncture and moxibustion combined with Chaihu Shugan Recipe can upregulate the expression of BDNF protein in brain tissue of rats.Conclusion Acupuncture combined with Chaihu Shugan Recipe has a better protective effect on cerebral ischemia reperfusion injury in rats,which may be related to the interaction of monoamine neurotransmitters and the up regulation of BDNF protein expression.