hsa-circ-0001862对舌鳞癌细胞表型的影响研究

Effect of hsa-circ-0001862 on the Phenotype of Tongue Squamous Cell

目的研究环状RNAhsa-circ-0001862对舌鳞癌细胞表型的影响及其对舌鳞癌发生发展的作用机制。方法构建hsa-circ-0001862质粒,采用双荧光素酶报告基因及qRT-PCR的方法来验证hsa-circ-0001862与miR-23a-3p的相互作用关系。靶向抑制hsa-circ-0001862后,利用CCK8实验以及集落形成实验分别检测舌鳞癌细胞的细胞活性及集落形成能力;通过划痕实验以及Transwell实验检测舌鳞癌细胞的迁移及侵袭能力;通过蛋白印迹实验检测凋亡相关蛋白,反映hsa-circ-0001862对舌鳞癌细胞凋亡能力的影响。结果hsa-circ-0001862与miR-23a-3p能够相互作用,在舌鳞癌细胞中两者的表达呈负相关;在Tca-8113细胞中,hsa-circ-0001862能够抑制细胞的增殖、迁移和侵袭的能力(P<0.01),并促进细胞的凋亡(P<0.01)。结论hsa-circ-0001862与miR-23a-3p相互作用,并且hsa-circ-0001862在舌鳞癌的发生发展中发挥着抑制的作用,hsa-circ-0001862或可成为治疗舌鳞癌的一个新的生物标记物和靶点。

Objective To investigate the effect of circular RNAhsa-circ-0001862 on the phenotype of tongue squamous cell carcinoma and its mechanism on the occurrence and development of tongue squamous cell carcinoma.Methods hsa-circ-0001862 plasmid was constructed,and the interaction relationship between hsa-circ-0001862 and miR-23a-3p was verified by double luciferase reporter gene and qRT-PCR.After targeted inhibition of hsa-circ-0001862,CCK8 assay and colony formation assay were used to detect the cell viability and colony formation ability of tongue squamous cell carcinoma cells.The migration and invasion ability of tongue squamous cell carcinoma cells were detected by scratch test and Transwell test.Apoptosis-related proteins were detected by Western blot to reflect the effect of hsa-circ-0001862 on the apoptosis of tongue squamous cell carcinoma cells.Results hsa-circ-0001862 and miR-23a-3p could interact,and their expression was negatively correlated in tonguesquamous cell,carcinoma cells.In Tca-8113 cells,hsa-circ-0001862 inhibited cell proliferation,migration and invasion(P<0.01),and promoted cell apoptosis(P<0.01).Conclusion hsa-circ-0001862 interacts with miR-23a-3p,and hsa-circ-0001862 plays an inhibitory role in the development of tongue squamous cell carcinoma.hsa-circ-0001862 maybe a new biomarker and target for the treatment of tonguesquamous cell carcinoma.