GC-MS/MS测定中药中13个挥发性有机化合物

Determination of 13 Volatile Organic Compounds in Traditional Chinese Medicine by GC-MS/MS

目的:建立一种测定经大孔树脂制备提取的中药中正己烷、苯、1,2-二氯乙烷、甲基丙烯酸甲酯、甲苯、氯苯、邻二甲苯、间二甲苯、对二甲苯、苯乙烯、1,2-二乙苯、二乙烯苯、萘13个挥发性有机化合物的通用方法。方法:采用气相色谱-质谱法测定供试品中13个挥发性有机化合物,以多反应监测模式采集数据。结果:对1批三七总皂苷、2批蒺藜皂苷提取物、2批银杏叶提取物、2批到手香提取物、2批到手香糖足愈合膏、1批益心酮片,合计10批样品进行测定。其中检出部分挥发性有机化合物,检测指标合格,安全风险较低。结论:建立的方法便捷、准确度高、灵敏度强,可用于经大孔树脂提取制备的中药提取物或制剂中挥发性有机化合物残留量的快速筛查和检测,可为中药质量安全监管提供参考。

Objective:To establish a general method for determining 13 volatile organic compounds(VOCs)in traditional Chinese medicine(TCM)prepared and extracted by macroporous resin,including n-hexane,benzene,1,2-dichloroethane,methyl methacrylate,toluene,chlorobenzene,o-xylene,m-xylene,p-xylene,styrene,1,2-diethylbenzene,divinylbenzene,and naphthalene.Methods:A gas chromatography-mass spectrometry(GC-MS/MS)method was used to determine the abovementioned VOCs in the samples,and data was collected under multi-reaction monitoring mode.Results:A total of ten batches of samples were determined,including one batch of Panax Notoginseng saponins,two batches of Tribulus terrestris saponin extract,two batches of Ginkgo biloba L.extract,two batches of Plectranthus amboinicus extract,two batches of Plectranthus amboinicus diabetic foot healing ointment,and one batch of Yixintong Tablets.Some VOCs were tested,and the test indexes were qualified,with low safety risk.Conclusion:The established method is convenient,accurate,and highly sensitive,and it can be used for rapid screening and detection of VOCs residues in TCM extracts or preparations prepared and extracted by macroporous resin,providing a reference for the quality and safety supervision of TCM.