miR-2467、miR-96-5p在妊娠期糖尿病中的临床意义

Clinical significance of miR-2467 and miR-96-5p in gestational diabetes mellitus

目的:探讨微小核糖核酸-2467(miR-2467)、miR-96-5p在妊娠期糖尿病(GDM)中的临床意义。方法:纳入108例GDM住院患者作为GDM组,另选取同期产检的糖耐量正常孕妇82例为对照组。比较2组血清miR-2467、miR-96-5p相对表达量以及空腹血糖(FBG)、餐后2 h血糖(2hFBG)、糖化血红蛋白(HbA1c)、胰岛素抵抗指数(HOMA-IR),分析miR-2467、miR-96-5p与上述血糖指标的相关性。采用Logistic多元回归模型分析GDM发生的危险因素,绘制受试者工作特征曲线,分析miR-2467、miR-96-5p评估GDM的曲线下面积(AUC)。结果:GDM组血清miR-2467表达、FBG、2hPG、HbA1c水平及HOMA-IR高于对照组,miR-96-5p表达低于对照组(P均<0.05)。Pearson相关性分析显示血清miR-2467表达与FBG、2hPG、HbA1c、HOMA-IR呈正相关,血清miR-96-5p表达与上述4项指标呈负相关(P均<0.05)。Logistic多元回归分析示孕早期BMI增长≥1.73kg/m^(2)、孕中期BMI增长≥4.88kg/m^(2)、糖尿病家族史、miR-2467≥3.44是GDM发生的危险因素,miR-96-5p≥1.95是GDM发生的保护因素(P<0.05)。血清miR-2467联合miR-96-5p评估GDM的AUC为0.865,敏感度、特异度分别为91.50%、81.50%。结论:miR-2467、miR-96-5p与GDM患者血糖指标及HOMA-IR存在相关性,对评估GDM具有一定意义。

Objective:To investigate the clinical significance of microRNA-2467(miR-2467)and miR-96-5p in gestational diabetes mellitus(GDM).Methods:A total of 108 inpatients with GDM were included as the GDM group,and 82 pregnant women with normal glucose tolerance during prenatal examinations were included as the control group.The relative expression levels of miR-2467,miR-96-5p,fasting plasma glucose(FPG),2-h postprandial blood glucose(2hPG),hemoglobin A1c(HbA1c)and homeostasis model assessment-insulin resistant index(HOMA-IR)were compared between the two groups,and the correlation between miR-2467,miR-96-5p and the above blood glucose indicators was analyzed.Logistic multivariate regression modeling was used to analyze the risk factors of GDM,and subject working characteristic curve was drawn to analyze the area under the curve(AUC)of miR-2467 and miR-96-5p to evaluate GDM.Results:The expression levels of serum miR-2467,FPG,2hPG,HbA1c and HOMA-IR in GDM group were higher than those in control group,and the expression of miR-96-5p was lower than that in control group(all P<0.05).Pearson linear correlation analysis showed that the expression of serum miR-2467 was positively correlated with FPG,2hPG,HbA1c and HOMA-IR,and the expression of serum miR-96-5p was negatively correlated with the above 4 indicators of blood glucose(all P<0.05).Logistic multiple regression analysis showed that the increase of BMI≥1.73 kg/m^(2) in early pregnancy,BMI increase≥4.88 kg/m^(2) in the second trimester,family history of diabetes and miR-2467≥3.44 were risk factors for GDM.miR-96-5p≥1.95 was a protective factor for GDM(P<0.05).The AUC of serum miR-2467 combined with miR-96-5p for evaluating GDM was 0.865,with sensitivity and specificity of 91.50%,and 81.50%,respectively.Conclusion:There is a correlation between miR-2467,miR-96-5p and blood glucose index and HOMA-IR in GDM,which has certain evaluation significance for GDM.