结直肠癌组织中错配修复蛋白的表达及其与临床病理特征的关系

Expression of mismatch repair proteins in colorectal cancer tissues and their relationship with clinical and pathological characteristics

目的分析结直肠癌组织中错配修复蛋白(MMR)的表达情况及与临床病理特征的关系,并探讨MMR检测在临床工作中的价值。方法收集2021年12月至2023年7月山东省济宁市第一人民医院胃肠外科经外科手术治疗的212例结肠癌及直肠癌患者的相关临床病历资料,分析MMR蛋白Mut L蛋白同系物1(MLH1)、Mut S蛋白同系物2(MSH2)、Mut S蛋白同系物6(MSH6)、减数分裂后分离蛋白2(PMS2)的表达情况并将错配蛋白缺失(dMMR)与其他临床病理数据进行分析。结果212例患者中存在MLH1缺失5例(2.4%),PMS2缺失10例(4.7%),MSH2与MSH6分别缺失2例(0.9%),MLH1、PMS2共同缺失5例(2.4%),MSH2、MSH6共同缺失2例(0.9%)。212例结直肠癌组织中dMMR 12例占5.7%;dMMR与结直肠癌患者的性别、年龄、分化程度、TNM分期、T分期、N分期、有无脉管癌栓及有无神经侵犯均无关;与肿瘤部位、组织学类型相关(P<0.05)。结论MMR表达缺失与肿瘤部位及组织学类型相关,通过对MMR的检测可对术后治疗方案提供指导并对判断癌症患者的预后有重要意义。

Objective To analyze the expression of mismatch repair proteins in colorectal cancer tissues and their relationship with clinical pathological features,and to explore the value of mismatch repair protein detec-tion in clinical work.Methods Clinical and pathological data of 212 patients with colon and rectal cancer who underwent surgical treatment in the Department of Gastroenterology at First People′s Hospital of Jining City from December 2021 to July 2023 were collected.The expression of MMR proteins MLH1,MSH2,MSH6,and PMS2 was analyzed,and dMMR was compared with other clinical and pathological data.Results Among 212 patients,there were 5 cases of MLH1 deficiency(2.4%),10 cases of PMS2 deficiency(4.7%),2 cases of MSH2 and MSH6 deficiency(0.9%),5 cases of MLH1 and PMS2 deficiency(2.4%),and 2 cases of MSH2 and MSH6 deficiency(0.9%).Among 212 cases of colorectal cancer,12 cases had dMMR,accounting for 5.7%;DMMR was not associ-ated with gender,age,degree of differentiation,TNM staging,T staging,N staging,presence or absence of vascular emboli,and presence or absence of nerve invasion in colorectal cancer patients;DMMR was related to tumor site and histological type(P<0.05).Conclusion The deletion of mismatch repair protein expression is significantly correlated with tumor location and histological type.Detection of mismatch repair proteins can provide guidance for postoperative treatment plans and is of great significance for predicting the prognosis of cancer patients.