MLH1和PMS2在胃癌组织中的缺失水平及价值分析

Deletion level and value analysis of MLH1 and PMS2 in gastric cancer tissues

目的探讨胃癌患者组织中错配修复(mismatch repair,MMR)蛋白MLH1、PMS2的缺失及表达水平,并分析高缺失者与患者临床特征的相关性。方法选取2014年5月至2022年3月青海省人民医院收治的275例胃癌患者为研究对象,回顾性收集患者MMR蛋白的表达资料,并结合临床特征进行统计学分析。结果在275例患者中,MLH1的缺失率为8.36%(23/275),PMS2的缺失率为18.18%(50/275),MLH1和PMS2的共同缺失率为7.27%(20/275)。MLH1的缺失率与PMS2的缺失率比较差异有统计学意义(P<0.05)。PMS2的缺失率与MLH1和PMS2的共同缺失率比较差异有统计学意义(P<0.05)。MLH1的缺失与胃癌患者的年龄、分化程度、Ki67指数、神经侵犯和Her-2状态有关(P<0.05);PMS2的缺失与胃癌患者的年龄、肿瘤部位和Ki67指数有关(P<0.05);MLH1和PMS2共同缺失与胃癌患者的年龄、肿瘤部位、肿瘤直径、分化程度、神经侵犯、Ki67指数和Her-2状态有关(P<0.05)。MLH1和PMS2在胃癌中表达呈正相关(r=0.539,P<0.001)。结论MLH1的缺失与胃癌患者较多的临床病理特征相关,MLH1、PMS2在胃癌中表达呈正相关。

Objective To investigate deletion and expression levels of mismatch repair(MMR)proteins MLH1 and PMS2 in gastric cancer patients,and analyze correlation between high deletion and clinical features of patients.Methods A total of 275 patients with gastric cancer treated in Qinghai Provincial People’s Hospital from May 2014 to March 2022 were selected as research subjects.Expression data of MMR protein were collected retrospectively,and statistical analysis was made based on clinical characteristics.Results In 275 cases,deletion rate of MLH1 was 8.36%(23/275),deletion rate of PMS2 was 18.18%(50/275),and deletion rate of both MLH1 and PMS2 was 7.27%(20/275).There was significant difference in deletion rate of MLH1 and PMS2(P<0.05).There was significant difference in deletion rate of PMS2 and both MLH1 and PMS2(P<0.05).Deletion of MLH1 was associated with age,differentiation degree,Ki67 index,nerve invasion and Her-2 status of gastric cancer patients(P<0.05).Deletion of PMS2 was associated with age,tumor location and Ki67 index of patients with gastric cancer(P<0.05).Simultaneous deletion of MLH1 and PMS2 was associated with age,tumor location,tumor diameter,differentiation degree,neural invasion,Ki67 index and Her-2 status of gastric cancer patients(P<0.05).Expression of MLH1 and PMS2 in gastric cancer was positively correlated(r=0.539,P<0.001).Conclusion Deletion of MLH1 is associated with more clinical pathological characteristics in patients with gastric cancer,and expression of MLH1 and PMS2 is positively correlated in gastric cancer.