血清CF-6、HSP-70表达对慢性心力衰竭患者病情及预后的评估价值

The Evaluation Value of Serum CF-6 and HSP-70 Expression for the Condition and Prognosis of Patients with Chronic Heart Failure

目的探究血清线粒体偶联因子-6(CF-6)、热休克蛋白70(HSP-70)表达对慢性心力衰竭(CHF)患者病情及预后的评估价值。方法选取2020年12月至2021年12月我院收治的CHF患者106例(CHF组),根据美国纽约心脏病协会(NYHA)心功能分级标准分为:NYHAⅡ级36例(Ⅱ级组),NYHAⅢ级38例(Ⅲ级组),NYHAⅣ级32例(Ⅳ级组);根据预后情况分为预后良好组54例,预后不良组52例;选择同期我院体检健康的106例志愿者为对照组。采用酶联免疫吸附测定(ELISA)法检测血清中CF-6、HSP-70的表达水平;多因素Logistic回归分析影响CHF预后的危险因素;受试者工作特征(ROC)曲线分析血清中CF-6、HSP-70对CHF预后的评估价值。结果与对照组相比,CHF组血清中CF-6、HSP-70表达水平显著升高(P<0.05)。CHF患者Ⅳ级组血清CF-6、HSP-70表达水平显著高于Ⅱ级组和Ⅲ级组,Ⅲ级组表达水平显著高于Ⅱ级组(P<0.05)。与预后良好组相比,预后不良组CF-6、HSP-70水平显著升高(P<0.05)。预后不良组血肌酐水平显著高于预后良好组(P<0.05)。多因素Logistic回归分析显示,CF-6、HSP-70、血肌酐表达水平是影响CHF预后的危险因素;ROC分析显示,血清CF-6、HSP-70水平联合预测CHF预后的AUC高于CF-6、HSP-70单独预测的AUC值(P<0.05)。结论CHF患者血清CF-6、HSP-70表达水平显著升高,并随着病情加重而升高,二者对CHF患者的预后评估有一定的临床价值。

Objective To investigate the value of serum mitochondrial coupling factoR6(CF-6)and heat shock protein 70(HSP-70)expression in evaluating the condition and prognosis of patients with chronic heart failure(CHF).Methods A total of 106 CHF patients admitted to ouRhospital from DecembeR2020 to DecembeR2021 were regarded as the CHF group,according to the New York Heart Association(NYHA)cardiac function grading standards,36 cases of NYHA gradeⅡwere classified as the gradeⅡgroup,38 cases of NYHA gradeⅢwere classified as the gradeⅢgroup,and 32 cases of NYHA gradeⅣwere classified as the gradeⅣgroup;according to the prognosis,54 cases were included into a good prognosis group and 52 cases into a pooRprognosis group;106 healthy volunteers who underwent physical examination during the same period in ouRhospital were regarded as the control group.Enzyme linked immunosorbent assay(ELISA)was applied to detect the expression levels of CF-6 and HSP-70 in serum;multivariate Logistic regression was applied to analyze the risk factors affecting the prognosis of CHF;and receiveRoperating characteristic(ROC)curve was applied to analyze the prognostic value of CF-6 and HSP-70 in serum foRCHF.Results The expression levels of CF-6 and HSP-70 in the serum of the CHF group were obviously increased compared to the control group(P<0.05).The expression levels of CF-6 and HSP-70 in serum of patients with CHF in gradeⅣgroup were obviously higheRthan those in gradeⅡandⅢgroups,and the expression levels in gradeⅢgroup were obviously higheRthan those in gradeⅡgroup(P<0.05).Compared with the good prognosis group,the levels of CF-6 and HSP-70 in the pooRprognosis group were obviously increased(P<0.05).Serum creatinine level in pooRprognosis group was significantly higheRthan that in good prognosis group(P<0.05).Multivariate Logistic regression analysis showed that CF-6,HSP-70 and serum creatinine expression were risk factors affecting the prognosis of CHF.ROC analysis showed that the AUC value predicted by serum CF-6 combined with HSP-70 foRthe prognosis of CHF was higheRthan that predicted by CF-6 and HSP-70 alone(P<0.05).Conclusion The expression levels of CF-6 and HSP-70 in the serum of CHF patients are obviously increased,and they increase with the aggravation of the disease.The two have certain clinical value in evaluating the prognosis of CHF patients.