基于网络药理学的中药组方治疗骨质疏松症的用药规律研究

Medication Rule of Traditional Chinese Medicine Prescriptions for Treating Osteoporosis Based on Network Pharmacology

目的 分析国家专利中药组方治疗骨质疏松症的用药规律并进行网络药理学分析。方法 在国家知识产权局网站中检索治疗慢骨质疏松的中药组方,规范药名后进行中药频数分析、性味归经分析,组方规律分析,药物靶点频数分析,k-means降维PPI分析、GO-BP生物学功能富集分析和KEGGPathway分析。结果 共纳入112项专利中药组方,涉及156味中药,其中淫羊藿的使用频次最高。药性以温性、平性、寒常见;五味多为甘、苦、辛;归经多归属肝经、肾经、脾经。关联规则分析提示常用的药对组合为牡蛎—淫羊藿,三七—当归,黄芪—淫羊藿等。核心靶点主要是AR、ESR1、ESR2、NR1I2、SHBG、VDR、NR3C1,GABRA家族和GABRG家族等。k-mean聚类分析PPI互作网络可降维成4类,与临床分型密切相关。GO和KEGG-Pathway分析表明中药复方主要通过神经活性配体-受体相互作用,氯化物跨膜转运,类固醇激素生物合成,碳水化合物的消化和多种物质成瘾的信号通路,通过消化吸收,神经信号和递质传递,类固醇的代谢,氯离子的转运等功能发挥防治骨质疏松症的作用。结论 结果表明中药治疗原发性骨质疏松症有规律可循,为中药治疗骨质疏松提供了理论依据,为中药的临床研究和基础研究提供了新的思路。

Objective To analyze the medication rule of nationally patented traditional Chinese medicine(TCM)prescriptions for treating osteoporosis and perform network pharmacology analysis.Methods TCM prescriptions for treating chronic osteoporosis were retrieved from the website of the China National Intellectual Property Administration.After standardizing the names of medicines,the frequency of TCM,their tropism of tastes and natures,prescription rules,and frequency of drug targets were analyzed,and k-means dimensionality reduction analysis of protein-protein interaction(PPI),gene ontology(GO)-backpropagation(BP)biological function enrichment analysis,and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis were conducted.Results A total of 112 patented TCM prescriptions involving 156 medicinal ingredients were included in the study,with epimedium being the most frequently used herb.The nature of the medicine was commonly warm,mild,or cold;the tastes were predominantly sweet,bitter,or spicy;and they mainly acted on the liver,kidney,and spleen channels.Association rule analysis indicated common drug pair combinations such as Concha Ostreae-epimedium,Notoginseng Radix-Radix Angelicae Sinensis,and Radix Astragali-epimedium.The core targets primarily included AR,ESR1,ESR2,NR1I2,SHBG,VDR,NR3C1,GABRA family,and GABRG family.The k-means clustering analysis of the PPI network could be reduced to four categories,closely related to clinical subtypes.GO and KEGG pathway analyses demonstrated that TCM compound prescription mainly exerted its effects on the prevention and treatment of osteoporosis through neuroactive ligand-receptor interactions,chloride transmembrane transport,steroid hormone biosynthesis,carbohydrate digestion,and signal pathways related to substance addiction,as well as functions such as digestion and absorption,neural signaling and neurotransmission,steroid metabolism,and chloride ion transport.ConclusionThe results indicate that TCM treatment for primary osteoporosis follows certain rules,providing a theoretical basis for the treatment of osteoporosis with TCM and offering new perspectives for clinical research and basic research on TCM.