基于生物信息数据分析C12ORF49在乳腺癌中的表达及其意义

Expression and significance of C12ORF49 in breast cancer based on Bioinformatic data

目的通过在线数据库分析12号染色体开放阅读框49(C12ORF49)在乳腺癌中的表达情况、临床意义及互作蛋白功能,为乳腺癌防治和预后预测提供新思路。方法使用Oncomine数据库及人类蛋白质图谱(HPA)数据库分析C12ORF49基因和蛋白在乳腺癌不同组织中的表达差异性,然后应用Kaplan-Meier Plotter数据库分析C12ORF49表达水平与不同病理特征乳腺癌患者预后的相关性,最后利用STRING数据库绘制C12ORF49互相作用蛋白网络图,并通过KOBAS生物分析平台进行KEGG功能富集分析。结果乳腺癌中C12ORF49的表达高于正常组织,乳腺癌C12ORF49 mRNA高表达患者的总生存时间(OS)及无远处转移生存时间(DMFS)更短,差异有统计学意义(P<0.05);C12ORF49可预测luminal B型乳腺癌患者的不良DMFS预后(P<0.05);C12ORF49对乳腺癌不良DMFS预后的影响可能独立于乳腺癌受体表达状态。C12ORF49可能通过NF-κB信号通路、TCR信号通路、血小板激活等生理过程(P<0.01,Corrected-P<0.01),促进乳腺癌进展。结论C12ORF49在乳腺癌组织中表达上调,其高表达水平对预测乳腺癌患者不良预后有重要意义,需进一步深入研究。

Objective To analyze the expression,clinical significance and interacting protein function of chromosome 12 open reading frame 49(C12ORF49)in breast cancer through online databases,and to provide a new approach for the prevention,treatment and prognosis of breast cancer.Methods The Oncomine database and the Human Protein Atlas(HPA)database were used to analyze the differential expression of C12ORF49 gene and protein in breast cancer,and the Kaplan-Meier Plotter database was applied to analyze the correlation between C12ORF49 expression levels and the prognosis of breast cancer patients with different pathological features,and finally the STRING database was employed to map the C12ORF49-related reciprocal protein network and KEGG functional enrichment analysis was performed in KOBAS bioanalysis platform to predict its biological function.Results C12ORF49 expression was higher in breast cancer tissues than that in normal tissues.Overall survival time(OS)and distant metastasis-free survival time(DMFS)were shorter in the breast cancer patients with high C12ORF49 mRNA expression than that in the breast cancer patients with low C12ORF49 mRNA expression,and the difference was statistically significant(P<0.05);C12ORF49 predicted poor DMFS prognosis in patients with Luminal B breast cancer(P<0.05).The effect of C12ORF49 on poor DMFS prognosis of breast cancer was probably independent of the expression profile of breast cancer receptor.These physiological processes such as NF-κB signaling pathway,TCR signaling pathway and platelet activation were associated with C12ORF49-boosted breast cancer(P<0.01,Corrected-P<0.01),promoted the progress of breast cancer.Conclusion C12ORF49 is upregulated in breast cancer tissues,and is important for the prognosis of breast cancer patients,which is worth the further study.