摘要
Herein,a new nanozyme/natural enzyme hybrid biosensor was established for ultrasensitive l-glutamic acid(L-Glu)detection.The Fe_(3)O_(4) nanoparticles with peroxidase-like activity and stability was used as a nanozyme and carrier for immobilizing l-Glutamate oxidase(GLOD)through Schiffff base reaction to construct a chem-enzyme cascade detector.The resultant Fe_(3)O_(4)-GOLD exhibited a wide linear range(10−500μM)and a low detection limit of 6.03μM for L-Glu detection.Furthermore,the Fe_(3)O_(4)-GOLD exhibited excellent pH stability,thermal stability,reusability and storage stability.After repeated nine cycles,Fe_(3)O_(4)-GOLD still retained 70%of its initial activity.Meanwhile,Fe_(3)O_(4)-GOLD maintained 50%of its initial activity after storage for 20 days,while free GLOD only retained 20%of its initial activity.This strategy of integrating biomimetic Fe 3 O4 and natural enzymes for cascade catalysis makes it possible to design an efficient and stable chemo-enzyme composite catalysts,which are promising for applications in biosensing and biomimetic catalysis.
基金
supported by the Open Project Program of State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology(project No.SKLFNS-KF-202203)
support from the Science and Technology Program of Tianjin,China(project No.20ZYJDJC00080).
