3种植物生长调节剂对桃儿七种子萌发特性的影响

Effects of Three Plant Growth Regulators on Seed Germination Characteristics of Sinopodophyllum hexandrum

目的:掌握珍稀藏药材桃儿七(Sinopodophyllum hexandrum)种子萌发特性及休眠解除方法。方法:以采集的野生桃儿七种子为研究对象,使用3种植物生长调节剂乙烯利(ETH)、褪黑素(MLT)、赤霉素(GA_(3)),对桃儿七种子萌发特性进行研究,设置不同浸种浓度、浸种时间探究对桃儿七种子萌发特性的影响。结果:ETH最佳处理条件为10 mg·L^(-1)浸种24 h,种子第45 d时萌发率达90.0%,萌发时滞为17 d;MLT最佳处理条件为50μmol·L^(-1)浸种12 h,种子第45 d时萌发率达83.3%,萌发时滞为16 d;GA_(3)最佳处理条件为200 mg·L^(-1)浸种24 h,种子第45 d时萌发率达80.0%,萌发时滞为18 d。三者相比,ETH对促进桃儿七种子萌发效果最显著。结论:桃儿七种皮坚硬,酸蚀组和对照组种子的吸水饱和时间明显不同;种子内含有萌发抑制性物质是导致桃儿七种子休眠的主要原因;3种植物生长调节剂对桃儿七种子萌发均有显著影响(P<0.05)。本研究探讨了打破桃儿七种子休眠并提高萌发率的有效方法,可为桃儿七规模化人工栽培提供科学指导及技术支撑。

Objective:To master the dormancy characteristics and the methods of dormancy release of Sinopodophyllum hexandrum seeds.Methods:Three plant growth regulators ethephon(ETH),melatonin(MLT)and gibberellin(GA3)were used to observe the effects of different soaking concentrations and soaking time on the germination characteristics of S.hexandrum seeds.Results:The optimal treatment conditions of ETH was 10 mg·L^(-1)soaking for 24 h,the germination rate reached 90.0% on the 45th day,and the germination delay was 17 d.The optimal treatment condition of MLT was soaked in 50μmol·L^(-1)for 12 h,the germination rate reached 83.3% on the 45th day,and the germination delay was 16 d.The optimal treatment condition of GA3 was 200 mg·L^(-1)soaking for 24 h,the germination rate reached 80.0% on the 45th day,and the germination delay was 18 d.The plant growth regulator ETH had the most significant effect on promoting the germination of S.hexandrum compared to the other two.Conclusion:The dense seed coat and the time of water saturation were significantly different between the acid-etching group and the control group.The main reason for seed dormancy was the existence of germination inhibitors in seeds.The three plant growth regulators had significant effects on the germination of S.hexandrum(P<0.05).This study explored the effective methods to break the dormancy of S.hexandrum seeds and improve the germination rate,which could provide scientific guidance and technical support for the large-scale artificial cultivation of S.hexandrum.