不同外源激素处理对翅柃种子发芽的影响

Effects of Different Exogenous Hormone Treatments on Seed Germination of Eurya alata

为了提高翅柃种子的发芽率,以翅柃种子为材料,通过采用不同外源激素浸种,以探索促进翅柃种子萌发的方法。对翅柃种子的表型性状进行测定;用TTC染色法测定种子生活力;选用不同浓度(50、100、150、200 mg/L)的赤霉素(GA 3)、吲哚乙酸(IAA)、萘乙酸(NAA)分别浸种1、2 h,对翅柃种子进行激素浸种处理,以清水浸种1、2 h为对照,在25℃恒温条件下观测翅柃种子的萌发情况。结果表明:翅柃种子呈三角形或不规则菱形,长0.8~1.0 mm,宽0.5~1.0 mm;翅柃种子生活力很低,仅为8%;翅柃种子经不同浓度激素处理后发芽率、发芽势、发芽时间有显著差异,NAA 150 mg/L浸种2 h发芽率7%,GA 3100 mg/L浸种时间1 h发芽率2%,IAA 200 mg/L浸种1 h发芽率4%,其余处理组合发芽率较低或均无发芽种粒。

In order to improve the germination rate of Eurya alata seeds,we used different exogenous hormones to immerse the seeds in order to explore ways to promote the germination of Eurya alata seeds.The seed viability of Eurya alata was determined by TTC staining,and different concentrations of gibberellin(GA 3),indole-3-acetic acid(IAA)and naphthylacetic acid(NAA)were used,seeds were soaked in water for 1 h and 2 h,respectively,and the germination of Eurya alata seeds was observed at 25℃.The results showed that the seeds were triangular or irregular rhombus,0.8-1.0 mm in length and 0.5-1.0 mm in width.The germination percentage,germination potential and germination time of Eurya alata seeds treated with different concentrations of hormones were significantly different.The germination percentage of seeds treated with NAA 150 mg/L for 2 h was 7%,and that of seeds treated with GA 3100 mg/L for 1 h was 2%,the germination rate of seeds soaked with IAA 200 mg/L for 1 h was 4%,and other treatments have lower germination rates or no seeds germinated.