检疫性害虫带木蠹象Pissodes castaneus(DeGeer)的鉴定

Identification of Pissodes castaneus(DeGeer),a quarantine pest

带木蠹象(Pissodes castaneus)是一种重要的林木入侵害虫,是我国进境植物检疫性有害生物。该虫原产欧洲各国,现已传入非洲和南美洲。国内尚无该虫的分布记录,2017年8月首次在海南洋浦口岸截获。带木蠹象主要为害松科植物的形成层和韧皮部,并在树皮下形成蛹室,可通过进境木材贸易进行远距离传播。笔者利用DNA条形码技术扩增目标样本线粒体CO I基因,与GenBank数据库以及BOLD数据库对比分析,并使用邻接法构建该类昆虫支序图,结果表明试验样品与带木蠹象P. castaneus聚为一支且99%以上同源性。DNA条形码技术为口岸快速检疫通关和入侵林业害虫的检测提供了便捷可能。

Pissodes castaneus is an important invasive forest pest and a phytosanitary pest in China.The insect pest is native to European countries and has been introduced into Africa and South America.There is no record of the distribution of the insect pest in China,and it was first intercepted at Yangpu Port in Hainan,China in August 2017.P.castaneus mainly infests the cambium and phloem of Pinaceae plants and form pupa chambers under the bark,and it can be spread over long distances through imported timber trade.The adults,larva and pupa of an insect were collected at Yangput Port in August 2017,and their mitochondrial COI genes were amplified by using DNA barcoding and compared with those in GenBank database and BOLD database.The phylogenetic tree of this insect was constructed by using the neighbor-joining algorithm.The results showed that the insect sample and P.castaneus were clustered into the same family and had more than 99%homology.This DNA barcoding method provides a scientific basis for rapid quarantine clearance and detection of invasive forest pests.