香草兰精油Pickering乳液制备、性质及功能性研究

Studies on Preparation,Properties and Functionality of Vanilla Essential Oil Pickering Emulsion

为对植物精油进行有效保护,控制其缓慢释放并扩大其应用范围,以香草兰精油为原料,通过剪切超声技术制备以辛烯基琥珀酸淀粉(OSA淀粉)为固体颗粒的Pickering乳液,测定其在贮藏期间的稳定性及香气释放速率,探究乳液消化过程中的变化,并对精油及乳液的抗炎性和MCF-7细胞抗增殖性进行对比。结果表明,香草兰精油Pickering乳液液滴表面光滑,颗粒完整,最小粒径为0.54μm。Pickering乳液可有效提高精油贮藏稳定性。与香草兰精油相比,乳液有效降低内部精油的香气释放率约100倍。Pickering乳液具有显著的抗消化能力,游离脂肪酸释放率在120 min内达到22.35%。香草兰精油制备Pickering乳液后依旧保持较高的抗炎活性,与未经处理的MCF-7细胞相比,二者均具有较强抗增殖作用。结论:用OSA淀粉稳定香草兰精油制备的Pickering乳液可有效增加精油溶解度,提高稳定性,扩大应用范围。本试验可为香草兰精油的应用,特别是功能性物质的应用提供理论依据。

In order to protect essential oils,control the release and expand the scope of application,vanilla essential oil was used as raw material to prepare Pickering emulsion in which the OSA starch was used as solid particle stabilizer by shear ultrasonic technology.The stability properties and aroma release rates were investigated to explore the changes in the emulsion digestion process,the anti-inflammatory and anti-proliferative properties of MCF-7 cells of Pickering emulsions and essential oil.The results showed that the droplets of Pickering emulsion had smooth surface and complete particles with the minimum particle size of 0.54μm.The storage stability of essential oils can be effectively improved by Pickering emulsion.The emulsion effectively reduced the aroma release rate of the internal essential oil by about 100 times.Pickering emulsion showed significant resistance to digestion,so that the free fatty acid release rate reaches 22.35%within 120 min.The vanilla essential oil still maintained high anti-inflammatory activities in the form of Pickering emulsion.Compared with untreated MCF-7 cells,the stronger anti-proliferative effects were both found in essential oil and Pickering emulsion.These results showed that the vanilla essential oil prepared with Pickering emulsion can effectively be increased for the solubility of essential oils,the stability,and the application scope.Therefore,this experiment can provide a theoretical basis for the application of vanilla essential oil,especially the application of functional medicine.