POT1、Dickkopf-1、PTEN在不同临床特征垂体瘤中的表达水平及其相关性分析

Expression Levels of POT1,Dickkopf-1 and PTEN in Pituitary Adenomas with Different Clinical Features and their Correlation

探讨端粒保护蛋白-1(POT1)、Dickkopf-1、第10号染色体缺失的磷酸酶和张力蛋白同源基因(PTEN)在不同临床特征垂体瘤中的表达水平及其相关性。回顾性选取54例2018年8月~2022年12月于本院行手术治疗的垂体瘤患者的病理组织标本作为病例组,另选63例同期收集的正常垂体组织标本作为对照组。比较病例组和对照组POT1、Dickkopf-1、PTEN信使核糖核酸(mRNA)表达水平,不同临床特征垂体瘤患者垂体瘤组织POT1、Dickkopf-1、PTEN蛋白表达情况,并用Pearson相关性分析垂体瘤患者垂体瘤组织POT1、Dickkopf-1、PTEN表达的相关性。病例组POT1 mRNA表达水平高于对照组(P<0.05),Dickkopf-1、PTEN mRNA表达水平低于对照组(P<0.05)。病理学分级中高分化、肿瘤分期Ⅲ~Ⅳ期、肿瘤直径≥2 cm、浸润深度≥6 cm、有血管间隙浸润、有淋巴结转移的垂体瘤患者垂体瘤组织POT1蛋白阳性率高于病理学分级低分化、肿瘤分期Ⅰ~Ⅱ期、肿瘤直径<2 cm、浸润深度<6 cm、无血管间隙浸润、无淋巴结转移的垂体瘤患者(P<0.05),垂体瘤组织Dickkopf-1、PTEN蛋白阳性率低于病理学分级低分化、肿瘤分期Ⅰ~Ⅱ期、肿瘤直径<2 cm、浸润深度<6 cm、无血管间隙浸润、无淋巴结转移的垂体瘤患者(P<0.05)。Pearson相关性分析结果显示,垂体瘤患者垂体瘤组织POT1蛋白与Dickkopf-1、PTEN蛋白均呈负相关(r=-0.713、-0.659,P<0.05);Dickkopf-1蛋白与PTEN蛋白呈正相关(r=0.547,P<0.05)。POT1在垂体瘤患者垂体瘤组织中呈高表达,Dickkopf-1、PTEN在垂体瘤患者垂体瘤组织中呈低表达,垂体瘤患者垂体瘤组织中POT1、Dickkopf-1、PTEN表达具有一定关联性,可能参与患者疾病进展过程。

To investigate the expression levels protection of telomeres 1(POT1),Dickkopf-1 and phosphatase and tensin homolog deleted on chromosome ten(PTEN)in pituitary adenomas with different clinical features and their correlation,the pathological tissue specimens of 54 patients with pituitary adenoma who underwent surgical treatment in the authors'hospital from August 2018 to December 2022 were retrospectively selected as the case group,and 63 normal pituitary tissue specimens collected at the same time were selected as the control group.The expression levels of POT1,Dickkopf-1,PTEN messenger ribonucleic acid(mRNA)between the case group and the control group were compared,the expression of POT1,Dickkopf-1,PTEN proteins in pituitary tumor tissues of patients with different clinical characteristics were compared,and the correlation of expression of POT1,Dickkopf-1 and PTEN in pituitary adenomas was analyzed by Pearson correlation.The expression level of POT1 mRNA in the the case group was higher than that in the control group(P<0.05),while the expression level of Dickkopf-1 and PTEN mRNA was lower than the control group(P<0.05).The positive rate of POT-1 protein of pituitary tumor tissue in patients with pituitary adenoma with high differentiation,stageⅢ~Ⅳ,tumor diameter≥2 cm,invasion depth≥6 cm,vascular space invasion and lymph node metastasis were higher than the patients with low differentiation,stageⅠ~Ⅱ,tumor diameter<2 cm,invasion depth<6 cm,no vascular space invasion and no lymph node metastasis(P<0.05),and the positive rate of Dickkopf-1 and PTEN protein of pituitary tumor tissue were lower than the patients with low differentiation,stageⅠ~Ⅱ,tumor diameter<2 cm,invasion depth<6 cm,no vascular space invasion and no lymph node metastasis(P<0.05).The results of Pearson correlation analysis showed that POT1 protein of pituitary tumor tissue was negatively correlated with Dickkopf-1 and PTEN protein(r=-0.713,-0.659,P<0.05).Dickkopf-1 protein was positively correlated with PTEN protein(r=0.547,P<0.01).POT1 was highly expressed in pituitary adenoma tissues,Dickkopf-1 and PTEN were low expressed in pituitary adenoma tissues.The expression of POT1,Dickkopf-1 and PTEN in pituitary adenoma tissues of patients with pituitary adenoma had certain correlation,which could participate in the disease progression.