基于嘌呤环检测Zn^(2+)的荧光探针及其应用

Fluorescent probe based on purine for detecting Zn^(2+)and its application

基于嘌呤-色酮的席夫碱,设计、合成并论述了一种新型的检测Zn^(2+)的开启式荧光探针(E)-3-((2-(8,9-二(萘-1-基)-9H-嘌呤-6-基)腙)甲基)-4H-铬-4-酮(DPHC).DPHC在DMSO/HEPES(v/v=9:1,pH=7.4)溶液中对Zn^(2+)表现出显著的选择性和快速响应,并且伴随着从无色到淡绿色的颜色变化.DPHC的发射强度与Zn^(2+)的浓度(1-5μmol/L)之间存在良好的线性关系(R^(2)=0.97992),其检出限为150.0 nmol/L.Job′s plot和1H-NMR进一步证明了DPHC对Zn^(2+)的传感机理.

A new“turn-on”fluorescent probe derived from purine-chromone Schiff base(E)-3-((2-(8,9-di(naphthalen-1-yl)-9H-purin-6-yl)hydrazono)methyl)-4H-chromen-4-one(DPHC)for detecting Zn^(2+)was designed,synthesized and evaluated.The DPHC exhibited remarkable selectivity and rapid response towards Zn^(2+)in DMSO/HEPES(v/v=9∶1,pH=7.4),accompanying with a significant color change from colorless to pale green.A good linear relationship(R^(2)=0.97992)was obtained between the emission intensity of DPHC and the concentration of Zn^(2+)(1-5μmol/L)with low detection limit of 150.0 nmol/L.The sensing mechanism of DPHC to Zn^(2+)was supported by the Job′s plot and 1 H-NMR.