LED光质配比对香菇菌丝生长及转色期生理活性的影响

Influence of LED light quality ratios on the physiological regulation ofLentimula edodes mycelial growth and color conversion stage

为了探究不同光质和光质配比对香菇菌丝生长及转色期的影响,该研究采用9种LED光质处理,以香菇‘L808’为试材,测定了香菇菌丝生长及转色期的生长势、转色特点及5种胞外酶活性,并采用线性回归和主成分分析方法对各指标进行评价。结果表明:在菌丝生长期,与黑暗处理(CK1)相比,红光处理下菌丝平均生长速率显著提高12.13%(P<0.05),羧甲基纤维素酶(carboxymethylcellulase,CMC酶)活性平均增加66.81%,而红蓝光比为3︰7时,菌丝平均生长速率显著降低2.62%(P<0.05),CMC酶活性平均降低10.46%。在菌丝转色期,与白光处理(CK2)相比,红蓝光7︰3处理第54天时菌丝完成转色,漆酶活性平均增加19.21%,而此时红光和黄光处理下的菌丝未转色,漆酶活性平均降低9.50%。菌丝生长期CMC酶活性与木聚糖酶活性、淀粉酶活性与漆酶和多酚氧化酶活性均呈正相关。菌丝转色期CMC酶活性与木聚糖酶活性、淀粉酶活性、漆酶活性呈正相关,淀粉酶活性与漆酶活性、多酚氧化酶活性呈正相关。综上所述,红光和红蓝光7︰3分别是菌丝生长期和转色期的适宜光质。该研究结果可为香菇生长的LED光质选择以及香菇工厂化栽培下的光环境控制提供理论依据。

This study aims to investigate the effects of different light qualities on mycelial growth and color conversion in Lentimula edodes factory cultivation.LED plant filler lamps were utilized to supplement the light for Lentimula edodes mycelial growth and color conversion stages.Mycelial growth and physiological indexes were measured to analyze the influence level of light quality on different growth stages of mycelia.Taking Lentimula edodes'L808'as the test material,eight treatments of light quality were set at 25℃,including red light,blue light,green light,yellow light,red-green light 1:9,redgreen light 6:4,red-blue light 3:7 red-blue light 7:3,and dark conditions were set as the control in the mycelial growth stage,while the white light was set as the control in the color conversion stage.The mycelial growth conditions were determined in the mycelial growth and color conversion stages.The dynamic patterns of five kinds of extracellular enzymes were determined in the growth of Lentimula edodes mycelia under different LED light quality environments using linear regression and principal component analysis.The results show that:In the mycelial growth phase,compared with dark treatment(CK1),the average mycelial growth rate was significantly increased by 12.13%(P<0.05)under red light treatment,and the activity of carboxymethylcellulase(CMC enzyme)was increased by 66.81%on average,while the ratio of red to blue was 3:7.The average growth rate of mycelia was significantly decreased by 2.62%(P<0.05),and the average CMC enzyme activity was decreased by 10.46%.In the mycelium color conversion stage,compared with the white light treatment(CK2),the average laccase activity increased by 19.21%when mycelium transformation was completed on the 54th day of red and blue light 7:3 treatment,while the average laccase activity decreased by 9.50%when mycelium was not transformed under red and yellow light treatment.The dynamics of extracellular enzyme activity showed that compared with CK1,the enzyme activity was not inhibited under each light treatment from day 25 to day 30 of mycelia growth.The activity of CMC enzyme was positively correlated with the activity of xylanase,and the activity of amylase was positively correlated with the activity of laccase and polyphenol oxidase.CMC enzyme activity was positively correlated with xylanase activity,amylase activity and laccase activity,while the amylase activity was positively correlated with laccase activity and polyphenol oxidase activity.The principal component analysis showed that the red light treatment was ranked the first in the mycelial growth stage,and red-blue light 7:3 treatment was ranked the first in the mycelial color conversion stage.Therefore,the red light was suitable for mycelial growth,and red-blue light 7:3 was suitable for mycelial color conversion in the factory production of Lentimula edodes.The finding can provide a theoretical basis for the selection of LED light quality during growth and the control of light environment under the factory cultivation of Lentimula edodes.