超声辅助酶法提取滇刺枣多酚工艺优化及抗氧化活性研究

Study on optimization of ultrasound assisted enzymatic extraction of polyphenols from Zizyphus mauritiana Lam.and its antioxidant activity

目的优化滇刺枣多酚(Zizyphus mauritiana Lam.polyphenols,ZMP)超声辅助酶法提取工艺条件并测定ZMP抗氧化性。方法以液料比、乙醇体积分数、超声温度、超声时间、超声功率、酶用量作为考察因素,在单因素实验考察基础上设计响应面法实验优化ZMP提取条件,并对ZMP清除DPPH自由基(DPPH·)、羟基自由基(·OH)的能力进行研究。结果ZMP超声辅助酶法提取最佳工艺条件为:液料比为40∶1(mL∶g)、超声温度为41℃、超声功率为304W、酶用量为4.1%(m/m)、乙醇体积分数为60%、超声时间为50min,此时,ZMP提取量平均值为(18.41±0.16)mg·g^(-1),接近预测值(18.33mg·g^(-1))。抗氧化性测定结果显示,当ZMP质量浓度为3.0μg·mL^(-1)时,对DPPH·、·OH的清除率分别为75%、72%,二者清除率均大于对照药品维生素C。结论响应面法实验优化的ZMP超声辅助酶法提取工艺稳定、可行,具有较强的抗氧化性,ZMP可作为一种抗氧化剂开发利用。

DBJECTIVE Optimize the ultrasound assisted enzymatic extraction process conditions of Zizyphus mauritiana Lam.polyphenols(ZMP)from Zizyphus mauritiana Lam.and determine the antioxidant activity of ZMP.METHOD Based on the single factor experimental investigation,response surface methodology was designed to optimize the extraction conditions of ZMP using liquid-solid ratio,ethanol volume fraction,ultrasound temperature,ultrasound time,ultrasound power,and enzyme dosage as factors.Study the ability of ZMP to scavenge DPPH radicals(DPPH·)and hydroxyl radicals(·OH).RESULTS The optimal extraction conditions of ZMP ultrasonic assisted enzymatic method were as follows:liquid-solid ratio of 40∶1(mL∶g),ultrasonic temperature of 41℃,ultrasonic power of 304W,enzyme dosage of 4.1%(m/m),ethanol volume fraction of 60%,ultrasonic time of 50min,under these conditions,the average extraction amount of ZMP was(18.41±0.16)mg·g^(-1),which was close to the predicted value(18.33mg·g^(-1)).The results of antioxidant test showed that the mass concentration of ZMP was 3.0μg·mL^(-1),the clearance rates of DPPH·,·OH were 75%and 72%,respectively,and both of them were higher than that of VC.CONCLUSION The ultrasonic assisted enzymatic extraction process of ZMP optimized by response surface methodology is stable and feasible,and ZMP has strong antioxidant activity,which can be developed and utilized as an antioxidant.