植物毛状根研究及应用

Research and Application of Plant Hairy Root

发根农杆菌侵染植物诱导产生分枝呈毛状、生长迅速的不定根,通常称为毛状根。毛状根具有生长快、遗传性稳定、不需要外源激素也能自主生长、能够合成次生代谢产物等特点,通过优化培养条件可以实现提高次生代谢物产量的目的。简要阐述了发根农杆菌诱导植物形成毛状根的机理,着重介绍了毛状根合成次级代谢产物的影响因素以及近5年毛状根在合成次生代谢产物、环境修复和植株再生方面的应用,并对毛状根培养过程中存在的问题进行总结,旨在为毛状根相关研究和应用提供参考。

After the infection of Agrobacterium rhizogenes,a large number of fast-growing adventitious roots were induced in plants,usually called hairy roots.Hairy roots were characterized by rapid growth,stable heredity,growth autonomously without the addition of exogenous hormones and synthesis of secondary metabolites.By optimizing the cultivation conditions,the goal of increasing the yield of secondary metabolites was achieved.The mechanism of hairy roots induced by Agrobacterium rhizogenes in plants was briefly explained in this paper.The influence factors of the synthesis of secondary metabolites in hairy roots and the application of hairy roots in the synthesis of secondary metabolites,environmental remediation,and plant regeneration in the past 5 years were emphatically introduced.The problems existing in the cultivation process of hairy roots were summarized,aiming to provide reference for the research and application in hairy roots.