硝基苯并噁二唑修饰的共轭增强型氟硼二吡咯类纳米光敏剂的合成及性能研究

Synthesis and Performance of NBD-Modified BODIPY Nano-Photosensitizerwith Enhanced Conjugate Structure

氟硼二吡咯(BODIPY)类荧光染料是常用的光动力治疗(PDT)/光热治疗(PTT)光敏剂,具备良好的光物理性质。将硝基苯并噁二唑(NBD)与聚乙二醇(PEG)化的BODIPY母核偶联,改造分子的共轭结构得到BDP-1,并自组装成纳米光敏剂BDP-1 NPs,利用粒度电位分析仪分析了BDP-1 NPs的粒径分布与Zeta电位,系统评价了BDP-1 NPs的光学性质、光热转换效率及对HeLa和MCF-7细胞的光暗毒性。结果表明,BDP-1 NPs的粒径约为90.0 nm,具有良好的分散性;680 nm激光(功率密度1.00 W·cm^(-2))照射下,BDP-1、BDP-1 NPs的单线态氧量子产率分别为0.51、0.28;BDP-1 NPs的光热转换效率为62.4%,具有较好的光热治疗效果,光稳定性较好;BDP-1 NPs对HeLa和MCF-7细胞的暗毒性很低,而在光照条件下显示出较好的光毒性。光敏剂BDP-1 NPs有望协同PDT/PTT用于癌症光疗,为光敏剂的开发提供了一定参考。

Fluoroboron dipyrrole(BODIPY)fluorescent dye is a common photosensitizer for photodynamic therapy(PDT)/photothermal therapy(PTT),which has good photophysical properties.We coupled nitrobenzoxadiazole(NBD)with PEG-functionalized BODIPY parent nucleus,and modified the conjugate structure of the molecule to obtain BDP-1,which self-assembled into nano-photosensitizer BDP-1 NPs.Moreover,we analyzed the particle size distribution and Zeta potential of BDP-1 NPs by particle size/potential analyzer.Furthermore,we systematically evaluated the optical properties,photothermal conversion efficiency,and photo-dark toxicity of BDP-1 NPs to HeLa and MCF-7 cells.The results show that the particle size of BDP-1 NPs is about 90.0 nm,and it has good dispersibility.Under 680 nm laser irradiation(power density of 1.00 W·cm^(-2)),the singlet oxygen quantum yields of BDP-1 and BDP-1 NPs are 0.51 and 0.28,respectively.The photothermal conversion efficiency of BDP-1 NPs is 62.4%,indicating that BDP-1 NPs has good photothermal therapeutic efficacy and good photostability.BDP-1 NPs shows low dark toxicity to HeLa and MCF-7 cells,while shows good photo toxicity under light conditions.The photosensitizer BDP-1 NPs is expected to be used for cancer phototherapy in coordination with PDT/PTT.This study provides a certain reference for the development of photosensitizers.