以酚醛树脂为前驱体合成氮掺杂中空碳球及其类酶活性研究

Synthesis of N-doped hollow carbon spheres with phenolic resin as precursor and their peroxidase-like activity

以间苯二酚甲醛树脂为前驱体,三聚氰胺为氮源,球形碳酸钙为模板剂,氢氧化钾为扩孔剂,通过硬模板法合成了氮掺杂中空碳球(NHCSs),通过扫描电子显微镜、场发射透射电子显微镜、X射线光电子能谱仪对NHCSs的微观形貌、表面元素组成及化学价态进行了表征,探究了NHCSs过氧化物模拟酶活性及反应体系中产生的活性氧中间体。结果表明:在弱酸性和H_(2)O_(2)存在的条件下,NHCSs可以快速地催化氧化3,3′,5,5′-四甲基联苯胺(TMB)生成蓝色产物;经过氢氧化钾扩孔处理后的NHCS-1对TMB和H_(2)O_(2)具有良好的亲和力;超氧自由基·O_(2)^(-)和羟基自由基·OH是主要的活性物种。采用简单快速的比色法检测H_(2)O_(2),线性范围为8~1000μmol/L,检测限为4.97μmol/L。

Using resorcinol formaldehyde resin as precursor,melamine as nitrogen source,spherical calcium carbonate(CaCO 3)as template agent and KOH as pore expanding agent,N-doped hollow carbon spheres(NHCSs)were synthesized by hard template method.The microscopic morphology,surface element composition and chemical valence states of NHCSs were characterized by scanning electron microscope,field emission transmission electron microscope and X-ray photoelectron spectrometer,and the activity of NHCSs peroxide-simulated enzyme and reactive oxygen species produced in the reaction system were investigated.The results showed that NHCSs could rapidly catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine(TMB)to produce a blue product under the condition of weak acidity and the presence of H_(2)O_(2).NHCS-1 after KOH reaming had a good affinity for TMB and H_(2)O_(2).The reactive oxygen intermediates produced in the reaction system were explored,and it was proved that·O_(2)^(-) and·OH were the main active species.A simple and rapid colorimetric method was used to detect H_(2)O_(2),with a linear range of 8~1000μmol/L.The detection limit was 4.97μmol/L.