高效液相色谱法在茶叶有机酸检测中的应用研究进展

Progress in the Application of High Performance Liquid Chromatography in the Detection of Organic Acids in Tea

茶叶中的有机酸是影响茶叶香气和滋味的主要成分之一,高效液相色谱法是食品中有机酸检测的常用方法,具有操作简单、灵敏度高、分离效果好等特点。文章综述了有机酸对茶叶风味品质的影响、高效液相色谱法目前能测定出的茶叶中有机酸种类以及采用高效液相色谱法测定茶叶中有机酸的研究进展,总结了测定茶叶中有机酸的高效液相色谱条件,包括检测波长、流动相、柱温和流速等,并对采用高效液相色谱法测定茶叶中有机酸存在的困难及该方法的应用前景进行了展望,以期促进茶叶中有机酸检测技术的发展,为茶叶的品质特征及保健功效研究提供指导,为茶叶中有机酸组分检测方法标准的制定提供参考。

Organic acids are the main components affecting tea aroma and taste.High performance liquid chromatography is a commonly used method for detecting organic acids in food,which has the characteristics of simple operation,high sensitivity,and good separation effect.The article reviewed the effects of organic acids on the flavor and quality of tea,the types of organic acids that could be determined in tea using high-performance liquid chromatography,as well as relative research progress.It summarized the conditions for determining organic acids in tea using high performance liquid chromatography,including the selection of detection wavelength,mobile phase,flow rate,and column temperature.The difficulties in using high performance liquid chromatography to determine organic acids in tea and the application prospects of the method were discussed,in order to promote the development of organic acid detection technology in tea.This paper provided guidance for the study of quality characteristics and health benefits of tea,and provided a reference for the formulation of detection method standards for organic acid components in tea.