CTRP2与主动脉夹层发生的相关性研究

The Correlation Between CTRP2 and Occurrence of Aortic Dissection

目的检测C1q肿瘤坏死因子相关蛋白2(CTRP2)在患者主动脉、小鼠主动脉以及小鼠主动脉血管平滑肌细胞中的表达水平,分析其与主动脉夹层(AD)发病之间的相关性。方法利用GEO数据库分析CTRP2在AD患者和非AD患者中的表达差异。选择2021年6月—2022年12月在武汉大学人民医院的6例确诊为急性Stanford A型AD患者的主动脉标本作为夹层组,6例心脏移植患者的主动脉作为对照组,通过Western blot法检测夹层组与对照组的CTRP2表达差异。将24只C57BL/6雄性3周龄野生型小鼠随机分为对照组和夹层组,夹层组用含有0.25%β-氨基丙腈的饲料喂养4周,取主动脉组织用石蜡包埋,标本切片后用免疫组织化学法检测CTRP2表达差异。细胞实验分为对照组和夹层组,分别用磷酸盐缓冲溶液和不同浓度的血管紧张素Ⅱ处理小鼠主动脉血管平滑肌细胞后提取细胞,用Western blot法检测CTRP2表达水平。结果免疫组织化学和Western blot法检测结果显示CTRP2在夹层组的患者主动脉、小鼠主动脉以及小鼠主动脉血管平滑肌细胞中的表达均显著升高。结论CTRP2蛋白表达水平在对照组和夹层组中的表达存在差异,与AD的发生具有相关性,有望成为治疗AD的新靶点。

Objective To detect the expression level of C1q tumor necrosis factor-related protein 2(CTRP2)in the aorta of patients,aorta of mouse and mouse aortic vascular smooth muscle cells,and to analyze its correlation with the pathogenesis of aortic dissection(AD).Methods The GEO database analyzed the difference in expression of CTRP2 in patients with and without AD.The aortic specimens of patients diagnosed with acute Stanford type A AD in Renmin Hospital of Wuhan University from June 2021 to December 2022 were selected as the dissection group(n=6),and the aorta of heart transplant patients was used as the control group(n=6),and the difference in CTRP2 expression between the dissection group and the control group was detected by Western blot method.Twenty-four C57BL/6 male 3-week-old wild-type mice were randomly classified into control group and dissection group,and the dissection group was fed with feed containing 0.25%β-aminopropionitrile for 4 weeks.The aorta tissue was embedded with paraffin,and the specimens were sectioned and detected the difference in CTRP2 expression by immunohistochemistry.The cells were divided into control group and dissection group,and mouse aortic vascular smooth muscle cells were extracted after treating with PBS and different concentrations of angiotensinⅡ.The expression level of CTRP2 was detected by Western blot method.Results The results of immunohistochemistry and Western blot showed that the expression of CTRP2 in the aorta of patients,aorta of mouse and mouse aortic vascular smooth muscle cells in the dissected group were significantly increased.Conclusion There are differences in the expression level of CTRP2 protein in the control group and the dissection group,which is correlated with the occurrence of AD,and is expected to become a new therapeutic target for AD.