摘要
CRISPR/Cas9技术的兴起,推动了生命科学各个领域的发展。随着对其认识的不断加深,人们进行了多重改进和优化,以适应不同的应用场景。在基因修饰小鼠模型制作中,CRISPR/Cas9技术的优化也带来了众多突破性进展。本文简要回顾了CRISPR/Cas9技术的发展历程,并从CRISPR/Cas9元件优化、条件敲除/敲入基因修饰小鼠模型的构建以及CRISPR/Cas9元件和HDR模板的递送系统层面总结了优化策略,展望该技术未来的发展。
CRISPR/Cas9 technology has driven the development of various fields in life science.With continuous deepening of its understanding,researchers have made multiple improvements and optimizations to adapt to various application scenarios.The optimization of CRISPR/Cas9 technology has also provided breakthroughs in the establishment of genetically modified mouse models.This article briefly reviews the development process of CRISPR/Cas9 technology and summarizes optimization strategies of CRISPR/Cas9,establishment of conditional knockout/knockin gene-modified mouse models,and delivery systems for CRISPR/Cas9 elements and HDR templates.
作者
王煜
吴勇
梁春南
范昌发
WANG Yu;WU Yong;LIANG Chunnan;FAN Changfa(Institute for Laboratory Animal Resources,National Institutes for Food and Drug Control(NIFDC),Beijing 102629,China)
出处
《中国比较医学杂志》
CAS
北大核心
2024年第5期152-158,共7页
Chinese Journal of Comparative Medicine
基金
国家重点研发计划(2021YFC2301700)。
关键词
CRISPR/Cas9
小鼠模型
条件敲除/敲入
递送
CRISPR/Cas9
mouse model
conditional knockout/knockin
delivery Conflicts of Interest:The authors declare no conflict of interest