5种不同的植物激素对白花前胡种子萌发的影响

Effect of 5 phytohormones on the germination of Peucedanum praeruptorum Dunn seeds

目的 研究生长素(IAA)、细胞分裂素(6-BA)、赤霉素(GA3)、脱落酸(ABA)和乙烯(ET)5种植物激素对白花前胡种子萌发的影响。方法 以宁国产并在室温放置12个月的白花前胡种子为材料,把5种不同的植物激素均配制成4个梯度浓度(1×10^(-1)、1×10^(-2)、1×10^(-3)、1×10^(-4)mg·mL^(-1)),对白花前胡种子进行浸种处理。通过培养皿萌发试验,分析种子的发芽率、根长和鲜重的变化情况。结果 在4个梯度范围内,6-BA对白花前胡种子的生长状态整体起抑制作用。把1×10^(-1)mg·mL^(-1)和1×10^(-2)mg·mL^(-1)设置为高浓度,1×10^(-3)mg·mL^(-1)和1×10^(-4)mg·mL^(-1)设置为低浓度。其中6-BA无论是高浓度还是低浓度均对白花前胡种子的生长状态整体起抑制作用。而GA3和ET在高浓度(1×10^(-1)mg·mL^(-1)和 1×10^(-2)mg·mL^(-1))及ABA和IAA在低浓度(1×10^(-3)mg·mL^(-1)和1×10^(-4)mg·mL^(-1))下均对白花前胡种子的萌发率、根长和鲜重都表达出明显的促进作用。结论 植物激素对白花前胡种子的生长发育均有不同程度的影响,其中ET在1×10^(-2)mg·mL^(-1)时,对白花前胡种子的生长发育影响最为显著,白花前胡种子的萌发率,最大根长和最大鲜重均高于其他组。

Objective To determine the effect of 5 phytohormones[growth hormone auxin(IAA),cytokinin(6-BA),gibberellin(GA3),abscisic acid(ABA)and ethylene(ET)]on seed germination of Peucedanum praeruptorum Dunn seeds was studied.Methods The experiment was conducted with Peucedanum praeruptorum Dunn seeds produced in Ningguo and left at room temperature for 12 months.Five different phytohormones were prepared with 4 gradient concentrations(1×10^(-1),1×10^(-2),1×10^(-3),and 1×10^(-4) mg·mL^(-1)),and the seeds of Peucedanum praeruptorum Dunn were imbibed.Changes in germination rate,root length and fresh weight of seeds in petri dish germination experiments were compared.Results Over a range of 4 gradients,6-BA overally inhibited the growth of Peucedanum praeruptorum Dunn seeds.In contrast,both GA3 and ET at high concentrations(1×10^(-1) mg·mL^(-1) and 1×10^(-2) mg·mL^(-1)),and ABA and IAA at low concentrations(1×10^(-3) mg·mL^(-1) and 1×10^(-4) mg·mL^(-1))at promoted the germination rate,root length,and fresh weight of Peucedanum praeruptorum Dunn seeds.Conclusion Phytohormones show different effects on the growth and development of Peucedanum praeruptorum Dunn seeds.ET at(1×10^(-2) mg·mL^(-1))shows the most obvious effect on the growth and development of Peucedanum praeruptorum Dunn seeds,reaching high germination rate,maximum root length and fresh weight.