樟属6种植物精油成分及其抑癌作用研究

The Chemical Compounds and Anti-tumor Activity of Six Essential Oils from Cinnamomum Schaeff

为探讨樟属植物精油成分及其抑制癌细胞活性,采用GC-MS和GC-FID对来自樟树、黄樟的6种精油成分进行了定性定量分析,并用CCK8法研究了6种精油对人胰腺癌细胞PANC-1和人肝癌细胞HUH7的抑制作用。结果表明,1号精油主要成分为柠檬醛(β-柠檬醛29.49%、α-柠檬醛35.88%),2号精油主要为α-蒎烯(18.53%)和4-松油醇(24.06%),3号精油主要为芳樟醇(94.52%),4号精油主要为1,8-桉叶油素(50.19%),5号精油主要为反式-橙花叔醇(66.00%),6号精油主要为甲基丁香酚(16.24%)和甲基异丁香酚(69.30%)。6种精油均表现出很强的抑制肿瘤细胞增殖活性,在同一浓度下,抑制肝癌细胞增殖活性显著高于抑制胰腺癌细胞增殖活性,同时1、5、6号精油对2种癌细胞的抑制率显著高于2、3、4号精油对2种癌细胞的抑制率。6种精油成分差异显著,均表现出很强的抑制肿瘤细胞增殖活性,是开发抗癌药物的潜在新原料。

In order to explore the essential oils of Cinnamomum Schaeff plants and their anti-tumor activities,the chemical components of six essential oils extracted from Cinnamomum camphora and Cinnamomum porrectum were qualitatively and quantitatively analyzed by GC-MS and GC-FID,and the inhibitory effects of six essential oils on human pancreatic cancer cell PANC-1 and humanhepatocarcinoma cell HUH7 were investigated using CCK8 method.The results showed that the main chemical component of sample No.1 was citral(withβ-citral content 29.49%andα-citral 35.88%),sample No.2 is mainlyα-pinene with 18.53%and 4-terpineol with 24.06%.Linalool,1,8-cineole,trans nerolidol predominated in sample No.3,No.4,No.5 respectively,with the content of 94.52%,50.19%and 66.00%.And sample No 6 was predominated by methyl eugenol(16.24%)and methyl isoeugenol(69.30%).The inhibitory effect on liver cancer cells was significantly higher than that on pancreatic cancer cells at the same content.In addition,the inhibitory rate of sample No.1,No.5,and No.6 on cancer cells was significantly higher than that of sample No.2,No.3,and No.4.The chemical components of the six essential oils are significantly different,all of which show strong inhibitory activity on tumor cell proliferation,and are potential raw materials for the development of anticancer drugs.