大肠埃希菌生物被膜表型、基因型与多重耐药的相关性分析

Analysis of the Correlation of Bacterial Biofilm Phenotype and Genotype with Multidrug Resistance in Escherichia Coli

【目的】探讨大肠埃希菌生物被膜表型、基因型与大肠埃希菌多重耐药的相关性。【方法】收集临床分离的230株大肠埃希菌,采用多重PCR法进行基因分型,采用改良结晶紫方法测定生物被膜形成能力,采用K-B法进行药敏试验。统计并分析大肠埃希菌耐药情况,大肠埃希菌生物被膜表型与多重耐药情况,大肠埃希菌基因型与多重耐药情况,大肠埃希菌生物被膜基因型与多重耐药的关系。【结果】大肠埃希菌对氨苄西林、头孢呋辛、环丙沙星耐药率均较高,对哌拉西林耐药率较低,对亚胺培南无耐药性;大肠埃希菌生物被膜表型分为强、中等、弱、无,分别占3.91%、31.30%、57.83%、6.96%,其中中等和弱占比较高,生物被膜形成能力越强,其多重耐药率越高;大肠埃希菌基因型中CTX-M-9基因型菌株数占比最高,为35.65%;Spearman相关性分析显示,大肠埃希菌生物被膜形成能力与多重耐药率呈正相关(P=0.012)。【结论】大肠埃希菌生物被膜形成能力与多重耐药率有关,各基因型对多种抗生素均表现为耐药,临床应合理应用抗生素。

【Objective】To explore the correlation of the bacterial biofilm phenotype and genotype with multidrug resistance in Escherichia coli.【Methods】A total of 230 clinical isolates of Escherichia coli were collected.Genotyping was performed using multiplex PCR,biofilm formation ability was determined using a modified crystal violet assay,and antibiotic susceptibility was tested using the Kirby-Bauer method.Statistical analysis was conducted on the antibiotic resistance patterns of Escherichia coli,the relationship between bacterial biofilm phenotype and multidrug resistance,and the relationship between Escherichia coli genotype and multidrug resistance.【Results】Escherichia coli showed high resistance rates to ampicillin,cefuroxime,and ciprofloxacin,but lower resistance to piperacillin and no resistance to imipenem.Biofilm phenotypes of Escherichia coli were classified as strong,moderate,weak and none,accounting for 3.91%,31.30%,57.83%and 6.96%,respectively.The common biofilm phenotypes in Escherichia coli were moderate and weak phenotypes.The stronger biofilm formation ability was associated with higher rate of multidrug resistance.The CTX-M-9 genotype was the most prevalent,accounting for 35.65%.Spearman correlation analysis showed that the biofilm formation ability of Escherichia coli was positively correlated with multidrug resistance rate(P=0.012).【Conclusion】The biofilm formation ability of Escherichia coli is associated with multidrug resistance.Different genotypes exhibit resistance to multiple antibiotics,highlighting the need for strict regulation of antibiotic use in clinical settings.