基于阀切换UHPLC-IC联用技术的实验教学探索

Exploration on Experiment Teaching of UHPLC-IC Based on Valve Switching Method

具有阀切换的超高效液相色谱-离子色谱联用(UHPLC-IC)技术适用于同时分析含有机物和无机阴离子的样品,该联用技术简化了前处理的过程,消除了复杂基体,对无机阴离子进行富集,实现有机物和无机阴离子的同时分析,提高了色谱分析效率。将阀切换UHPLC-IC联用技术引入到本科生仪器分析实验课堂中,加深了学生对新型分析技术独特优势的理解,提高了本科生解决复杂实际问题的能力,拓宽和提升了仪器分析实验的教学内涵,有利于创新性和综合性人才的培养。

The technology of ultrahigh performance liquid chromatography(UHPLC)and ion chromatography(IC)with valve switching method is suitable for the simultaneous analysis of samples containing organic compounds and inorganic anions,which simplifies the pretreatment process,eliminates the complex matrix,enriches inorganic anions,realizes the simultaneous analysis of organic compounds and inorganic anions,and improves the efficiency of chromatographic analysis.The technology of ultrahigh performance liquid chromatography and ion chromatography with valve switching method is introduced into the undergraduate instrument analysis experiment class,which deepens the students’understanding of the special advantages of the new technology,improves the undergraduates’ability to solve complex practical problems,widens the field and enriches the content of instrument analysis experiment.Of course,it is conducive to the cultivation of innovative and comprehensive talents.